Role of glutathione in the prevention of cataractogenesis in rat lenses

Naseem H. Ansari; Satish K. Srivastava

doi:10.3109/02713688209011630

Role of glutathione in the prevention of cataractogenesis in rat lenses

Naseem H. Ansari, Satish K. Srivastava

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A new method has been developed for the conjugation of rat lens glutathione by 1-chloro-2,4-dinitrobenzene (CDNB). This reaction is catalysed by glutathione S-transferase present in the lens. One milliliter of 1 mM CDNB per two rat lenses conjugates more than 95% of the lens GSH in 30 min at 37d̀. Lenses incubated with 1 mM CDNB for 30 min, followed by incubation in the culture medium without CDNB remained apparently clear for up to 24 hrs. The CDNB treated lenses were more susceptible to protein precipitation (cataract formation) when challenged with oxidants such as hydrogen peroxide and superoxide anions.

Original language	English (US)
Pages (from-to)	271-275
Number of pages	5
Journal	Current Eye Research
Volume	2
Issue number	4
DOIs	https://doi.org/10.3109/02713688209011630
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Role of glutathione in the prevention of cataractogenesis in rat lenses",

abstract = "A new method has been developed for the conjugation of rat lens glutathione by 1-chloro-2,4-dinitrobenzene (CDNB). This reaction is catalysed by glutathione S-transferase present in the lens. One milliliter of 1 mM CDNB per two rat lenses conjugates more than 95% of the lens GSH in 30 min at 37{\`d}. Lenses incubated with 1 mM CDNB for 30 min, followed by incubation in the culture medium without CDNB remained apparently clear for up to 24 hrs. The CDNB treated lenses were more susceptible to protein precipitation (cataract formation) when challenged with oxidants such as hydrogen peroxide and superoxide anions.",

author = "Ansari, {Naseem H.} and Srivastava, {Satish K.}",

note = "Funding Information: ACKNOWLEDGMENTS This investigation was supported by PHS grants EY 01677 and EY 02260, awarded by the National Eye Institute, DHHS.",

year = "1982",

doi = "10.3109/02713688209011630",

language = "English (US)",

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journal = "Current Eye Research",

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T1 - Role of glutathione in the prevention of cataractogenesis in rat lenses

AU - Ansari, Naseem H.

AU - Srivastava, Satish K.

N1 - Funding Information: ACKNOWLEDGMENTS This investigation was supported by PHS grants EY 01677 and EY 02260, awarded by the National Eye Institute, DHHS.

PY - 1982

Y1 - 1982

N2 - A new method has been developed for the conjugation of rat lens glutathione by 1-chloro-2,4-dinitrobenzene (CDNB). This reaction is catalysed by glutathione S-transferase present in the lens. One milliliter of 1 mM CDNB per two rat lenses conjugates more than 95% of the lens GSH in 30 min at 37d̀. Lenses incubated with 1 mM CDNB for 30 min, followed by incubation in the culture medium without CDNB remained apparently clear for up to 24 hrs. The CDNB treated lenses were more susceptible to protein precipitation (cataract formation) when challenged with oxidants such as hydrogen peroxide and superoxide anions.

AB - A new method has been developed for the conjugation of rat lens glutathione by 1-chloro-2,4-dinitrobenzene (CDNB). This reaction is catalysed by glutathione S-transferase present in the lens. One milliliter of 1 mM CDNB per two rat lenses conjugates more than 95% of the lens GSH in 30 min at 37d̀. Lenses incubated with 1 mM CDNB for 30 min, followed by incubation in the culture medium without CDNB remained apparently clear for up to 24 hrs. The CDNB treated lenses were more susceptible to protein precipitation (cataract formation) when challenged with oxidants such as hydrogen peroxide and superoxide anions.

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Role of glutathione in the prevention of cataractogenesis in rat lenses

Abstract

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