Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells

Giovanni Suarez, Johanna C. Sierra, Michelle L. Kirtley, Ashok Chopra

Research output: Contribution to journalArticle

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Abstract

Recently, we reported that the type 6 secretion system (T6SS) of Aeromonas hydrophila SSU plays an important role in bacterial virulence in a mouse model, and immunization of animals with the T6SS effector haemolysin co-regulated protein (Hcp) protected them against lethal infections with wild-type bacteria. Additionally, we showed that the mutant bacteria deleted for the vasH gene within the T6SS gene cluster did not express the hcp gene, while the vasK mutant could express and translocate Hcp, but was unable to secrete it into the extracellular milieu. Both of these A. hydrophila SSU mutants were readily phagocytosed by murine macrophages, pointing to the possible role of the secreted form of Hcp in the evasion of the host innate immunity. By using the ΔvasH mutant of A. hydrophila, our in vitro data showed that the addition of exogenous recombinant Hcp (rHcp) reduced bacterial uptake by macrophages. These results were substantiated by increased bacterial virulence when rHcp was added along with the ΔvasH mutant in a septicaemic mouse model of infection. Analysis of the cytokine profiling in the intraperitoneal lavage as well as activation of host cells after 4 h of infection with the ΔvasH mutant supplemented with rHcp indicated that this T6SS effector inhibited production of pro-inflammatory cytokines and induced immunosuppressive cytokines, such as interleukin-10 and transforming growth factor-β, which could circumvent macrophage activation and maturation. This mechanism of innate immune evasion by Hcp possibly inhibited the recruitment of cellular immune components, which allowed bacterial multiplication and dissemination in animals, thereby leading to their mortality.

Original languageEnglish (US)
Pages (from-to)3678-3688
Number of pages11
JournalMicrobiology
Volume156
Issue number12
DOIs
StatePublished - Dec 2010

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Aeromonas hydrophila
Hemolysin Proteins
Cytokines
Proteins
Virulence
Infection
Macrophages
Bacteria
Immune Evasion
Bacterial Proteins
Macrophage Activation
Therapeutic Irrigation
Transforming Growth Factors
Immunosuppressive Agents
Multigene Family
Phagocytosis
Innate Immunity
Interleukin-10
Genes
Immunization

ASJC Scopus subject areas

  • Microbiology

Cite this

Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. / Suarez, Giovanni; Sierra, Johanna C.; Kirtley, Michelle L.; Chopra, Ashok.

In: Microbiology, Vol. 156, No. 12, 12.2010, p. 3678-3688.

Research output: Contribution to journalArticle

Suarez, Giovanni ; Sierra, Johanna C. ; Kirtley, Michelle L. ; Chopra, Ashok. / Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. In: Microbiology. 2010 ; Vol. 156, No. 12. pp. 3678-3688.
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