Role of human placental efflux transporter P-glycoprotein in the transfer of buprenorphine, levo-α-acetylmethadol, and paclitaxel

Ilona A. Nekhayeva, Tatiana Nanovskaya, Gary Hankins, Mahmoud Ahmed

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32 Citations (Scopus)

Abstract

This study examines the role of placental P-glycoprotein (P-gp) in the transfer of buprenorphine (BUP) and L-α-acetylmethadol (LAAM) across the dually perfused human placental lobule. BUP (10 ng/mL) and LAAM (35 ng/mL) were perfused in the maternal-to-fetal direction. The following kinetic parameters were determined: fetal transfer rate (TRf), maternal clearance (Clm), and clearance index (Clindex). The opiates were perfused in the presence of P-gp inhibitor GF120918 (experimental group) and in its absence (control group). The kinetic parameters for the control group were set at 100% and were as follows for LAAM in the experimental group: TR f, 123 ± 20%, Clm 116 ± 23%, and Cl index 123 ± 22% (P < 0.05). The corresponding parameters for BUP were not different from controls. The data indicate that LAAM, but not BUP, is extruded by the efflux transporter P-gp. Therefore, it is reasonable to assume that the activity of P-gp could be one of the factors affecting the extent of fetal exposure to LAAM during pregnancy.

Original languageEnglish (US)
Pages (from-to)423-430
Number of pages8
JournalAmerican Journal of Perinatology
Volume23
Issue number7
DOIs
StatePublished - Oct 2006

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Methadyl Acetate
Buprenorphine
P-Glycoprotein
Paclitaxel
Opiate Alkaloids
Mothers
Control Groups
Pregnancy

Keywords

  • Buprenorphine
  • Human placenta
  • Levo-α-acetylmethadol
  • P-glycoprotein

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

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title = "Role of human placental efflux transporter P-glycoprotein in the transfer of buprenorphine, levo-α-acetylmethadol, and paclitaxel",
abstract = "This study examines the role of placental P-glycoprotein (P-gp) in the transfer of buprenorphine (BUP) and L-α-acetylmethadol (LAAM) across the dually perfused human placental lobule. BUP (10 ng/mL) and LAAM (35 ng/mL) were perfused in the maternal-to-fetal direction. The following kinetic parameters were determined: fetal transfer rate (TRf), maternal clearance (Clm), and clearance index (Clindex). The opiates were perfused in the presence of P-gp inhibitor GF120918 (experimental group) and in its absence (control group). The kinetic parameters for the control group were set at 100{\%} and were as follows for LAAM in the experimental group: TR f, 123 ± 20{\%}, Clm 116 ± 23{\%}, and Cl index 123 ± 22{\%} (P < 0.05). The corresponding parameters for BUP were not different from controls. The data indicate that LAAM, but not BUP, is extruded by the efflux transporter P-gp. Therefore, it is reasonable to assume that the activity of P-gp could be one of the factors affecting the extent of fetal exposure to LAAM during pregnancy.",
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AU - Hankins, Gary

AU - Ahmed, Mahmoud

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AB - This study examines the role of placental P-glycoprotein (P-gp) in the transfer of buprenorphine (BUP) and L-α-acetylmethadol (LAAM) across the dually perfused human placental lobule. BUP (10 ng/mL) and LAAM (35 ng/mL) were perfused in the maternal-to-fetal direction. The following kinetic parameters were determined: fetal transfer rate (TRf), maternal clearance (Clm), and clearance index (Clindex). The opiates were perfused in the presence of P-gp inhibitor GF120918 (experimental group) and in its absence (control group). The kinetic parameters for the control group were set at 100% and were as follows for LAAM in the experimental group: TR f, 123 ± 20%, Clm 116 ± 23%, and Cl index 123 ± 22% (P < 0.05). The corresponding parameters for BUP were not different from controls. The data indicate that LAAM, but not BUP, is extruded by the efflux transporter P-gp. Therefore, it is reasonable to assume that the activity of P-gp could be one of the factors affecting the extent of fetal exposure to LAAM during pregnancy.

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