Role of interferon antagonist activity of rabies virus phosphoprotein in viral pathogenicity

Naoto Ito, Gregory W. Moseley, Danielle Blondel, Kenta Shimizu, Caitlin L. Rowe, Yuki Ito, Tatsunori Masatani, Keisuke Nakagawa, David A. Jans, Makoto Sugiyama

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The fixed rabies virus (RV) strain Nishigahara kills adult mice after intracerebral inoculation, whereas the chicken embryo fibroblast cell-adapted strain Ni-CE causes nonlethal infection in adult mice. We previously reported that the chimeric CE(NiP) strain, which has the phosphoprotein (P protein) gene from the Nishigahara strain in the genetic background of the Ni-CE strain, causes lethal infection in adult mice, indicating that the P gene is responsible for the different pathogenicities of the Nishigahara and Ni-CE strains. Previous studies demonstrated that RV P protein binds to the interferon (IFN)-activated transcription factor STAT1 and blocks IFN signaling by preventing its translocation to the nucleus. In this study, we examine the molecular mechanism by which RV P protein determines viral pathogenicity by comparing the IFN antagonist activities of the Nishigahara and Ni-CE P proteins. The results, obtained from both RV-infected cells and cells transfected to express P protein only, show that Ni-CE P protein is significantly impaired for its capacity to block IFN-activated STAT1 nuclear translocation and, consequently, inhibits IFN signaling less efficiently than Nishigahara P protein. Further, it was demonstrated that a defect in the nuclear export of Ni-CE P protein correlates with a defect in its ability to cause the mislocalization of STAT1. These data provide the first evidence that the capacity of the RV P protein to inhibit STAT1 nuclear translocation and IFN signaling correlates with the viral pathogenicity.

Original languageEnglish (US)
Pages (from-to)6699-6710
Number of pages12
JournalJournal of Virology
Volume84
Issue number13
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Rabies virus
phosphoproteins
Phosphoproteins
interferons
Interferons
Virulence
antagonists
pathogenicity
mice
STAT1 Transcription Factor
Cell Nucleus Active Transport
Infection
lethal genes
infection
genetic background
Genes
fibroblasts
Chickens
embryo (animal)
genes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Ito, N., Moseley, G. W., Blondel, D., Shimizu, K., Rowe, C. L., Ito, Y., ... Sugiyama, M. (2010). Role of interferon antagonist activity of rabies virus phosphoprotein in viral pathogenicity. Journal of Virology, 84(13), 6699-6710. https://doi.org/10.1128/JVI.00011-10

Role of interferon antagonist activity of rabies virus phosphoprotein in viral pathogenicity. / Ito, Naoto; Moseley, Gregory W.; Blondel, Danielle; Shimizu, Kenta; Rowe, Caitlin L.; Ito, Yuki; Masatani, Tatsunori; Nakagawa, Keisuke; Jans, David A.; Sugiyama, Makoto.

In: Journal of Virology, Vol. 84, No. 13, 07.2010, p. 6699-6710.

Research output: Contribution to journalArticle

Ito, N, Moseley, GW, Blondel, D, Shimizu, K, Rowe, CL, Ito, Y, Masatani, T, Nakagawa, K, Jans, DA & Sugiyama, M 2010, 'Role of interferon antagonist activity of rabies virus phosphoprotein in viral pathogenicity', Journal of Virology, vol. 84, no. 13, pp. 6699-6710. https://doi.org/10.1128/JVI.00011-10
Ito, Naoto ; Moseley, Gregory W. ; Blondel, Danielle ; Shimizu, Kenta ; Rowe, Caitlin L. ; Ito, Yuki ; Masatani, Tatsunori ; Nakagawa, Keisuke ; Jans, David A. ; Sugiyama, Makoto. / Role of interferon antagonist activity of rabies virus phosphoprotein in viral pathogenicity. In: Journal of Virology. 2010 ; Vol. 84, No. 13. pp. 6699-6710.
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