Role of interferon in streptococcal infection in the mouse

Douglas A. Weigent, Timothy L. Huff, Johnny Peterson, G. John Stanton, Samuel Baron

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In previous studies, we have shown the rapid in vitro induction of IFNγ from human T cells by highly purified peptic extracts of M proteins from Streptococcus pyogenes. The present report extends these in vitro studies and shows that a mixture of both α/β and γ IFN were present in spleen cell homogenates after in vivo treatment with M protein wild-type (M +) or mutant (M-) S. pyogenes strains. The levels of bacterial-induced IFN were found to be greater in M+ treated animals. Additional studies in vivo showed that pretreatment of mice with heat-killed M+ S. pyogenes organisms significantly protected mice to pneumococcal infection compared to similarly treated M- or control animals (P < 0.001) Further, antibodies to mouse IFNα/β and antibodies specific to a synthetic N-terminal peptide of mouse IFNγ enhanced the death of animals due to pneumococcal infection and blocked the protection observed in animals previously treated with heat-killed M+ organisms. Most importantly, treatment of mice with either type of IFN alone enhanced the survival of mice to levels similar to that observed by treatment with M+ organisms (P < 0.05). The results strongly suggest that IFN can play a crucial role, directly or indirectly, in controlling infection by Streptococcus pneumoniae and perhaps other streptococci.

Original languageEnglish (US)
Pages (from-to)399-407
Number of pages9
JournalMicrobial Pathogenesis
Volume1
Issue number4
DOIs
StatePublished - 1986

Fingerprint

Streptococcal Infections
Interferons
Pneumococcal Infections
Streptococcus pyogenes
Hot Temperature
Antibodies
Streptococcus
Digestion
Proteins
Spleen
T-Lymphocytes
Peptides

Keywords

  • alpha/beta
  • gamma
  • Interferon
  • interferon antibodies in vivo
  • M protein
  • streptococci

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Role of interferon in streptococcal infection in the mouse. / Weigent, Douglas A.; Huff, Timothy L.; Peterson, Johnny; Stanton, G. John; Baron, Samuel.

In: Microbial Pathogenesis, Vol. 1, No. 4, 1986, p. 399-407.

Research output: Contribution to journalArticle

Weigent, Douglas A. ; Huff, Timothy L. ; Peterson, Johnny ; Stanton, G. John ; Baron, Samuel. / Role of interferon in streptococcal infection in the mouse. In: Microbial Pathogenesis. 1986 ; Vol. 1, No. 4. pp. 399-407.
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