Role of interleukin-10 in the suppression of the immunological response in mice exposed to staphylococcal enterotoxin

B. G. Haskó, L. Virág, G. Egnaczyk, A. L. Salzman, Csaba Szabo

Research output: Contribution to journalArticle

Abstract

Staphylococcal enterotoxin B (SEB), a bacterial superantigen, activates the immune system resulting in a burst of pro- and anti-inflammatory cytokines. Using IL-10 deficient C57BL/6 (IL-10 KO) mice, we studied the role of endogenous IL-10 in the regulation of immune response to SEB. SEB (100 μg) induced the release of IL-10 in control C57BL/6 (IL-10 WT) mice, but not in the KO counterparts. SEB-evoked plasma levels of tumor necrosis factor-α, IL-1β, IL-2, IL-6, IL-12, and interferon-γ were significantly higher in the IL-10 KO mice than in WT animals. The release of macrophage inflammatory proteins -1α, and -2 were also enhanced in the IL-10 KO mice. Further, upon SEB challenge, mice deficient in IL-10 produced higher levels of nitric oxide than the WT animals. IL-10 deficiency resulted in a marked enhancement of the SEB-induced apoptosis of thymocytes. Finally, IL-10 KO mice were more susceptible to SEB-induced lethal shock than their WT controls. Thus, IL-10 plays an important immunoregulatory role in response to a superantigenic stimulus, by dampening of the shock-inducing inflammatory response and early activation-induced cell death elicited by SEB.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

Enterotoxins
enterotoxins
interleukin-10
Interleukin-10
immune response
mice
Shock
Animals
superantigens
Chemokine CXCL2
Macrophage Inflammatory Proteins
Superantigens
thymocytes
staphylococcal enterotoxin B
tumor necrosis factors
Immune system
interleukin-12
interleukin-1
Cell death
interferons

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Role of interleukin-10 in the suppression of the immunological response in mice exposed to staphylococcal enterotoxin. / Haskó, B. G.; Virág, L.; Egnaczyk, G.; Salzman, A. L.; Szabo, Csaba.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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