TY - JOUR
T1 - Role of intramitochondrial nitric oxide in rat heart and kidney during hypertension
AU - Aguilera-Aguirre, Leopoldo
AU - González-Hernández, Juan Carlos
AU - Pérez-Vázquez, Victoriano
AU - Ramírez, Joel
AU - Clemente-Guerrero, Mónica
AU - Villalobos-Molina, Rafael
AU - Saavedra-Molina, Alfredo
N1 - Funding Information:
This work was supported partially by the CONACYT Grant (29609-N), (28553-M to R.V.M) and by the CIC-UMSNH (2.5). L.A.-A. is a CONACYT Fellow (115536). We are indebted to Dr S. Uribe for the use of some of his lab facilities and to Mr Julio Sánchez for measuring blood pressure.
PY - 2002
Y1 - 2002
N2 - Nitric oxide (NO) is an important reactive molecule in many organisms. A mitochondrial nitric oxide synthase has been described; however, the role of NO in this organelle is not yet fully clear. We tested the effect of intramitochondrial NO on various functions from spontaneously hypertensive rats (SHR) and their normotensive genetic control. Wistar-Kyoto (WKY) rats. While the stimulation of intramitochondrial NOS increased calcium- and phosphate-induced permeability transition pore opening, its inhibition partially prevented it, without affecting membrane potential. Matrix free calcium and the pH decreased with NOS inhibition. Basal [NO] was lower in SHR than in WKY. Our data suggest that intramitochondrial NO plays an important role in mitochondrial regulation during hypertension.
AB - Nitric oxide (NO) is an important reactive molecule in many organisms. A mitochondrial nitric oxide synthase has been described; however, the role of NO in this organelle is not yet fully clear. We tested the effect of intramitochondrial NO on various functions from spontaneously hypertensive rats (SHR) and their normotensive genetic control. Wistar-Kyoto (WKY) rats. While the stimulation of intramitochondrial NOS increased calcium- and phosphate-induced permeability transition pore opening, its inhibition partially prevented it, without affecting membrane potential. Matrix free calcium and the pH decreased with NOS inhibition. Basal [NO] was lower in SHR than in WKY. Our data suggest that intramitochondrial NO plays an important role in mitochondrial regulation during hypertension.
KW - Hypertension
KW - Mitochondria
KW - Nitric oxide
KW - Nitric oxide synthase
UR - http://www.scopus.com/inward/record.url?scp=0036238070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036238070&partnerID=8YFLogxK
U2 - 10.1016/S1567-7249(02)00002-8
DO - 10.1016/S1567-7249(02)00002-8
M3 - Article
C2 - 16120294
AN - SCOPUS:0036238070
SN - 1567-7249
VL - 1
SP - 413
EP - 423
JO - Mitochondrion
JF - Mitochondrion
IS - 5
ER -