Role of intramitochondrial nitric oxide in rat heart and kidney during hypertension

Leopoldo Aguilera-Aguirre; Juan Carlos González-Hernández; Victoriano Pérez-Vázquez; Joel Ramírez; Mónica Clemente-Guerrero; Rafael Villalobos-Molina; Alfredo Saavedra-Molina

doi:10.1016/S1567-7249(02)00002-8

Role of intramitochondrial nitric oxide in rat heart and kidney during hypertension

Leopoldo Aguilera-Aguirre
, Juan Carlos González-Hernández
, Victoriano Pérez-Vázquez
, Joel Ramírez
, Mónica Clemente-Guerrero
, Rafael Villalobos-Molina
, Alfredo Saavedra-Molina

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Nitric oxide (NO) is an important reactive molecule in many organisms. A mitochondrial nitric oxide synthase has been described; however, the role of NO in this organelle is not yet fully clear. We tested the effect of intramitochondrial NO on various functions from spontaneously hypertensive rats (SHR) and their normotensive genetic control. Wistar-Kyoto (WKY) rats. While the stimulation of intramitochondrial NOS increased calcium- and phosphate-induced permeability transition pore opening, its inhibition partially prevented it, without affecting membrane potential. Matrix free calcium and the pH decreased with NOS inhibition. Basal [NO] was lower in SHR than in WKY. Our data suggest that intramitochondrial NO plays an important role in mitochondrial regulation during hypertension.

Original language	English (US)
Pages (from-to)	413-423
Number of pages	11
Journal	Mitochondrion
Volume	1
Issue number	5
DOIs	https://doi.org/10.1016/S1567-7249(02)00002-8
State	Published - 2002
Externally published	Yes

Keywords

Hypertension
Mitochondria
Nitric oxide
Nitric oxide synthase

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Cell Biology

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10.1016/S1567-7249(02)00002-8

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title = "Role of intramitochondrial nitric oxide in rat heart and kidney during hypertension",

abstract = "Nitric oxide (NO) is an important reactive molecule in many organisms. A mitochondrial nitric oxide synthase has been described; however, the role of NO in this organelle is not yet fully clear. We tested the effect of intramitochondrial NO on various functions from spontaneously hypertensive rats (SHR) and their normotensive genetic control. Wistar-Kyoto (WKY) rats. While the stimulation of intramitochondrial NOS increased calcium- and phosphate-induced permeability transition pore opening, its inhibition partially prevented it, without affecting membrane potential. Matrix free calcium and the pH decreased with NOS inhibition. Basal [NO] was lower in SHR than in WKY. Our data suggest that intramitochondrial NO plays an important role in mitochondrial regulation during hypertension.",

keywords = "Hypertension, Mitochondria, Nitric oxide, Nitric oxide synthase",

author = "Leopoldo Aguilera-Aguirre and Gonz{\'a}lez-Hern{\'a}ndez, \{Juan Carlos\} and Victoriano P{\'e}rez-V{\'a}zquez and Joel Ram{\'i}rez and M{\'o}nica Clemente-Guerrero and Rafael Villalobos-Molina and Alfredo Saavedra-Molina",

note = "Funding Information: This work was supported partially by the CONACYT Grant (29609-N), (28553-M to R.V.M) and by the CIC-UMSNH (2.5). L.A.-A. is a CONACYT Fellow (115536). We are indebted to Dr S. Uribe for the use of some of his lab facilities and to Mr Julio S{\'a}nchez for measuring blood pressure.",

year = "2002",

doi = "10.1016/S1567-7249(02)00002-8",

language = "English (US)",

volume = "1",

pages = "413--423",

journal = "Mitochondrion",

issn = "1567-7249",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Role of intramitochondrial nitric oxide in rat heart and kidney during hypertension

AU - Aguilera-Aguirre, Leopoldo

AU - González-Hernández, Juan Carlos

AU - Pérez-Vázquez, Victoriano

AU - Ramírez, Joel

AU - Clemente-Guerrero, Mónica

AU - Villalobos-Molina, Rafael

AU - Saavedra-Molina, Alfredo

N1 - Funding Information: This work was supported partially by the CONACYT Grant (29609-N), (28553-M to R.V.M) and by the CIC-UMSNH (2.5). L.A.-A. is a CONACYT Fellow (115536). We are indebted to Dr S. Uribe for the use of some of his lab facilities and to Mr Julio Sánchez for measuring blood pressure.

PY - 2002

Y1 - 2002

N2 - Nitric oxide (NO) is an important reactive molecule in many organisms. A mitochondrial nitric oxide synthase has been described; however, the role of NO in this organelle is not yet fully clear. We tested the effect of intramitochondrial NO on various functions from spontaneously hypertensive rats (SHR) and their normotensive genetic control. Wistar-Kyoto (WKY) rats. While the stimulation of intramitochondrial NOS increased calcium- and phosphate-induced permeability transition pore opening, its inhibition partially prevented it, without affecting membrane potential. Matrix free calcium and the pH decreased with NOS inhibition. Basal [NO] was lower in SHR than in WKY. Our data suggest that intramitochondrial NO plays an important role in mitochondrial regulation during hypertension.

AB - Nitric oxide (NO) is an important reactive molecule in many organisms. A mitochondrial nitric oxide synthase has been described; however, the role of NO in this organelle is not yet fully clear. We tested the effect of intramitochondrial NO on various functions from spontaneously hypertensive rats (SHR) and their normotensive genetic control. Wistar-Kyoto (WKY) rats. While the stimulation of intramitochondrial NOS increased calcium- and phosphate-induced permeability transition pore opening, its inhibition partially prevented it, without affecting membrane potential. Matrix free calcium and the pH decreased with NOS inhibition. Basal [NO] was lower in SHR than in WKY. Our data suggest that intramitochondrial NO plays an important role in mitochondrial regulation during hypertension.

KW - Hypertension

KW - Mitochondria

KW - Nitric oxide

KW - Nitric oxide synthase

UR - https://www.scopus.com/pages/publications/0036238070

UR - https://www.scopus.com/pages/publications/0036238070#tab=citedBy

U2 - 10.1016/S1567-7249(02)00002-8

DO - 10.1016/S1567-7249(02)00002-8

M3 - Article

C2 - 16120294

AN - SCOPUS:0036238070

SN - 1567-7249

VL - 1

SP - 413

EP - 423

JO - Mitochondrion

JF - Mitochondrion

IS - 5

ER -

Role of intramitochondrial nitric oxide in rat heart and kidney during hypertension

Abstract

Keywords

ASJC Scopus subject areas

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