Role of lipid aldehydes in cataractogenesis

4-hydroxynonenal-induced cataract

Naseem Ansari, Lifei Wang, Satish Srivastava

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Free radicals have extremely short half-lives and they readily oxidize lipids and initiate an autocatalytic chain reaction of lipid peroxidation, which leads to the formation of lipid peroxides. The lipid peroxides undergo degradation to form metastable lipid aldehydes such as 4-hydroxynonenal (HNE). We have shown earlier that under hyperglycemia, lipid peroxides increase; and aldose reductase, an enzyme that reduces glucose to sorbitol, efficiently reduces HNE. The purpose of the present studies was thus to investigate the role of HNE in hyperglycemic cataract and understand the mechanism(s) of its prevention by antioxidants and aldose reductase inhibitors. HNE and hyperglycemic cataract were developed by culturing rat lenses in TC-199 medium containing 50 μM lINE and 50 mM glucose, respectively. The effect of an antioxidant, trolox, and an aldose reductase inhibitor, sorbinil, on the progression of HNE and hyperglycemic cataract, evaluated by digital image analysis, was followed for 8 and 9 days, respectively. In lenses cultured with HNE, the decrease in transmitted light was 43, 65, and 87% on Days 3, 5, and 8, respectively. Trolox ameliorated the HNE cataract, whereas sorbinil accelerated the progression of HNE cataract and prevented the progression of hyperglycemic cataract. It is concluded that lINE formed under hyperglycemia may play a pivotal role in diabetic cataractogenesis.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalBiochemical and Molecular Medicine
Volume58
Issue number1
DOIs
StatePublished - Jun 1996

Fingerprint

Aldehydes
Cataract
Lipids
Aldehyde Reductase
Lipid Peroxides
Hyperglycemia
Lenses
Antioxidants
Glucose
Sorbitol
4-hydroxy-2-nonenal
Image analysis
Lipid Peroxidation
Free Radicals
Rats
Light
Degradation
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Role of lipid aldehydes in cataractogenesis : 4-hydroxynonenal-induced cataract. / Ansari, Naseem; Wang, Lifei; Srivastava, Satish.

In: Biochemical and Molecular Medicine, Vol. 58, No. 1, 06.1996, p. 25-30.

Research output: Contribution to journalArticle

@article{10393d686bbc432dab98836d258fc732,
title = "Role of lipid aldehydes in cataractogenesis: 4-hydroxynonenal-induced cataract",
abstract = "Free radicals have extremely short half-lives and they readily oxidize lipids and initiate an autocatalytic chain reaction of lipid peroxidation, which leads to the formation of lipid peroxides. The lipid peroxides undergo degradation to form metastable lipid aldehydes such as 4-hydroxynonenal (HNE). We have shown earlier that under hyperglycemia, lipid peroxides increase; and aldose reductase, an enzyme that reduces glucose to sorbitol, efficiently reduces HNE. The purpose of the present studies was thus to investigate the role of HNE in hyperglycemic cataract and understand the mechanism(s) of its prevention by antioxidants and aldose reductase inhibitors. HNE and hyperglycemic cataract were developed by culturing rat lenses in TC-199 medium containing 50 μM lINE and 50 mM glucose, respectively. The effect of an antioxidant, trolox, and an aldose reductase inhibitor, sorbinil, on the progression of HNE and hyperglycemic cataract, evaluated by digital image analysis, was followed for 8 and 9 days, respectively. In lenses cultured with HNE, the decrease in transmitted light was 43, 65, and 87{\%} on Days 3, 5, and 8, respectively. Trolox ameliorated the HNE cataract, whereas sorbinil accelerated the progression of HNE cataract and prevented the progression of hyperglycemic cataract. It is concluded that lINE formed under hyperglycemia may play a pivotal role in diabetic cataractogenesis.",
author = "Naseem Ansari and Lifei Wang and Satish Srivastava",
year = "1996",
month = "6",
doi = "10.1006/bmme.1996.0028",
language = "English",
volume = "58",
pages = "25--30",
journal = "Molecular Genetics and Metabolism",
issn = "1096-7192",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Role of lipid aldehydes in cataractogenesis

T2 - 4-hydroxynonenal-induced cataract

AU - Ansari, Naseem

AU - Wang, Lifei

AU - Srivastava, Satish

PY - 1996/6

Y1 - 1996/6

N2 - Free radicals have extremely short half-lives and they readily oxidize lipids and initiate an autocatalytic chain reaction of lipid peroxidation, which leads to the formation of lipid peroxides. The lipid peroxides undergo degradation to form metastable lipid aldehydes such as 4-hydroxynonenal (HNE). We have shown earlier that under hyperglycemia, lipid peroxides increase; and aldose reductase, an enzyme that reduces glucose to sorbitol, efficiently reduces HNE. The purpose of the present studies was thus to investigate the role of HNE in hyperglycemic cataract and understand the mechanism(s) of its prevention by antioxidants and aldose reductase inhibitors. HNE and hyperglycemic cataract were developed by culturing rat lenses in TC-199 medium containing 50 μM lINE and 50 mM glucose, respectively. The effect of an antioxidant, trolox, and an aldose reductase inhibitor, sorbinil, on the progression of HNE and hyperglycemic cataract, evaluated by digital image analysis, was followed for 8 and 9 days, respectively. In lenses cultured with HNE, the decrease in transmitted light was 43, 65, and 87% on Days 3, 5, and 8, respectively. Trolox ameliorated the HNE cataract, whereas sorbinil accelerated the progression of HNE cataract and prevented the progression of hyperglycemic cataract. It is concluded that lINE formed under hyperglycemia may play a pivotal role in diabetic cataractogenesis.

AB - Free radicals have extremely short half-lives and they readily oxidize lipids and initiate an autocatalytic chain reaction of lipid peroxidation, which leads to the formation of lipid peroxides. The lipid peroxides undergo degradation to form metastable lipid aldehydes such as 4-hydroxynonenal (HNE). We have shown earlier that under hyperglycemia, lipid peroxides increase; and aldose reductase, an enzyme that reduces glucose to sorbitol, efficiently reduces HNE. The purpose of the present studies was thus to investigate the role of HNE in hyperglycemic cataract and understand the mechanism(s) of its prevention by antioxidants and aldose reductase inhibitors. HNE and hyperglycemic cataract were developed by culturing rat lenses in TC-199 medium containing 50 μM lINE and 50 mM glucose, respectively. The effect of an antioxidant, trolox, and an aldose reductase inhibitor, sorbinil, on the progression of HNE and hyperglycemic cataract, evaluated by digital image analysis, was followed for 8 and 9 days, respectively. In lenses cultured with HNE, the decrease in transmitted light was 43, 65, and 87% on Days 3, 5, and 8, respectively. Trolox ameliorated the HNE cataract, whereas sorbinil accelerated the progression of HNE cataract and prevented the progression of hyperglycemic cataract. It is concluded that lINE formed under hyperglycemia may play a pivotal role in diabetic cataractogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0030175946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030175946&partnerID=8YFLogxK

U2 - 10.1006/bmme.1996.0028

DO - 10.1006/bmme.1996.0028

M3 - Article

VL - 58

SP - 25

EP - 30

JO - Molecular Genetics and Metabolism

JF - Molecular Genetics and Metabolism

SN - 1096-7192

IS - 1

ER -