Role of microglia in the dissemination of zika virus from mother to fetal brain

Pei Xu; Chao Shan; Tiffany J. Dunn; Xuping Xie; Hongjie Xia; Junling Gao; Javier Allende Labastida; Jing Zou; Paula P. Villarreal; Caitlin R. Schlagal; Yongjia Yu; Gracie Vargas; Shannan L. Rossi; Nikolaos Vasilakis; Pei-Yong Shi; Scott C. Weaver; Ping Wu

doi:10.1371/journal.pntd.0008413

Role of microglia in the dissemination of zika virus from mother to fetal brain

Pei Xu, Chao Shan, Tiffany J. Dunn, Xuping Xie, Hongjie Xia, Junling Gao, Javier Allende Labastida, Jing Zou, Paula P. Villarreal, Caitlin R. Schlagal, Yongjia Yu, Gracie Vargas, Shannan L. Rossi, Nikolaos Vasilakis, Pei-Yong Shi, Scott C. Weaver, Ping Wu

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22 Scopus citations

Abstract

Global Zika virus (ZIKV) outbreaks and their link to microcephaly have raised major public health concerns. However, the mechanism of maternal-fetal transmission remains largely unknown. In this study, we determined the role of yolk sac (YS) microglial progenitors in a mouse model of ZIKV vertical transmission. We found that embryonic (E) days 6.5-E8.5 were a critical window for ZIKV infection that resulted in fetal demise and microcephaly, and YS microglial progenitors were susceptible to ZIKV infection. Ablation of YS microglial progenitors significantly reduced the viral load in both the YS and the embryonic brain. Taken together, these results support the hypothesis that YS microglial progenitors serve as “Trojan horses,” contributing to ZIKV fetal brain dissemination and congenital brain defects.

Original language	English (US)
Article number	e0008413
Pages (from-to)	1-21
Number of pages	21
Journal	PLoS neglected tropical diseases
Volume	14
Issue number	7
DOIs	https://doi.org/10.1371/journal.pntd.0008413
State	Published - Jul 2020

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Infectious Diseases

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Xu, P., Shan, C., Dunn, T. J., Xie, X., Xia, H., Gao, J., Labastida, J. A., Zou, J., Villarreal, P. P., Schlagal, C. R., Yu, Y., Vargas, G., Rossi, S. L., Vasilakis, N., Shi, P.-Y., Weaver, S. C., & Wu, P. (2020). Role of microglia in the dissemination of zika virus from mother to fetal brain. PLoS neglected tropical diseases, 14(7), 1-21. Article e0008413. https://doi.org/10.1371/journal.pntd.0008413

Xu, P, Shan, C, Dunn, TJ, Xie, X , Xia, H, Gao, J, Labastida, JA, Zou, J, Villarreal, PP, Schlagal, CR, Yu, Y , Vargas, G , Rossi, SL , Vasilakis, N, Shi, P-Y, Weaver, SC & Wu, P 2020, 'Role of microglia in the dissemination of zika virus from mother to fetal brain', PLoS neglected tropical diseases, vol. 14, no. 7, e0008413, pp. 1-21. https://doi.org/10.1371/journal.pntd.0008413

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abstract = "Global Zika virus (ZIKV) outbreaks and their link to microcephaly have raised major public health concerns. However, the mechanism of maternal-fetal transmission remains largely unknown. In this study, we determined the role of yolk sac (YS) microglial progenitors in a mouse model of ZIKV vertical transmission. We found that embryonic (E) days 6.5-E8.5 were a critical window for ZIKV infection that resulted in fetal demise and microcephaly, and YS microglial progenitors were susceptible to ZIKV infection. Ablation of YS microglial progenitors significantly reduced the viral load in both the YS and the embryonic brain. Taken together, these results support the hypothesis that YS microglial progenitors serve as “Trojan horses,” contributing to ZIKV fetal brain dissemination and congenital brain defects.",

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Role of microglia in the dissemination of zika virus from mother to fetal brain

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