Role of nerve growth factor in oxidant-antioxidant balance and neuronal injury. I. Stimulation of hydrogen peroxide resistance

G. R. Jackson, L. Apffel, K. Werrbach-Perez, J. R. Perez-Polo

    Research output: Contribution to journalArticle

    154 Citations (Scopus)

    Abstract

    The nerve growth factor protein (NGF) regulates neuronal cell death during the development of embryonic sensory and sympathetic neurons in the peripheral nervous system (PNS). NGF protects the rat pheochromocytoma line PC12, a useful model of NGF responsive peripheral neurons, from hydrogen peroxide, which interacts with ferrous iron to generate hydroxyl radicals. Exogenous catalase provides protection, whereas superoxide dismutase (SOD) has no effect on neuronal survival when PC12 cells are challenged with hydrogen peroxide. NGF treatment of PC12 cells increases the activity of catalase. NGF protection from hydrogen peroxide is partially abolished by aminotriazole (Az), a low molecular weight catalase inhibitor. Taken together, these data are consistent with the hypothesis that NGF protects from peroxidative events and consequent cell death via an induction of free radical detoxifying mechanisms, such as catalase activity.

    Original languageEnglish (US)
    Pages (from-to)360-368
    Number of pages9
    JournalJournal of Neuroscience Research
    Volume25
    Issue number3
    StatePublished - 1990

    Fingerprint

    Nerve Growth Factor
    Oxidants
    Hydrogen Peroxide
    Antioxidants
    Catalase
    Wounds and Injuries
    Proteins
    PC12 Cells
    Cell Death
    Amitrole
    Peripheral Nervous System
    Pheochromocytoma
    Sensory Receptor Cells
    Hydroxyl Radical
    Superoxide Dismutase
    Free Radicals
    Embryonic Development
    Iron
    Molecular Weight
    Neurons

    Keywords

    • catalase
    • cell death
    • free radicals
    • NGF
    • regeneration

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Role of nerve growth factor in oxidant-antioxidant balance and neuronal injury. I. Stimulation of hydrogen peroxide resistance. / Jackson, G. R.; Apffel, L.; Werrbach-Perez, K.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 25, No. 3, 1990, p. 360-368.

    Research output: Contribution to journalArticle

    Jackson, G. R. ; Apffel, L. ; Werrbach-Perez, K. ; Perez-Polo, J. R. / Role of nerve growth factor in oxidant-antioxidant balance and neuronal injury. I. Stimulation of hydrogen peroxide resistance. In: Journal of Neuroscience Research. 1990 ; Vol. 25, No. 3. pp. 360-368.
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