Role of nerve growth factor in oxidant-antioxidant balance and neuronal injury. II. A conditioning lesion paradigm

G. R. Jackson, K. Werrbach-Perez, J. R. Perez-Polo

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    The PC12 rat pheochromocytoma cell line is a nerve growth factor (NGF) responsive line that is protected by NGF from the toxic effects of hydrogen peroxide induced peroxidation. In part, NGF protection of PC12 cells acts through a shift in oxidant-antioxidant metabolism by the enhancement of catalase activity. When PC12 cells are used in a conditioning lesion paradigm to study the effects of an initial sublethal peroxidative insult on subsequent responses to injury, a low dose conditioning lesion protects even in the absence of NGF. The magnitude of the protective effect exerted by the conditioning lesion, however, is augmented in the presence of NGF, since a significant cytoprotective effect is observed over a wide range of H2O2 concentrations. Neuronal injury due to treatment with a high dose of H2O2 (5 mM) has a cytotoxic effect that cannot be prevented by NGF treatment and is, in itself, not conditioning in nature. This in vitro model system lends itself to the development of explanations regarding the salutory effects of conditioning lesions at the molecular level.

    Original languageEnglish (US)
    Pages (from-to)369-374
    Number of pages6
    JournalJournal of Neuroscience Research
    Volume25
    Issue number3
    StatePublished - 1990

    Fingerprint

    Nerve Growth Factor
    Oxidants
    Antioxidants
    PC12 Cells
    Wounds and Injuries
    Poisons
    Catalase
    Hydrogen Peroxide

    Keywords

    • cell death
    • conditioning lesion
    • hydrogen peroxide
    • nerve injury
    • NGF

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Role of nerve growth factor in oxidant-antioxidant balance and neuronal injury. II. A conditioning lesion paradigm. / Jackson, G. R.; Werrbach-Perez, K.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 25, No. 3, 1990, p. 369-374.

    Research output: Contribution to journalArticle

    Jackson, G. R. ; Werrbach-Perez, K. ; Perez-Polo, J. R. / Role of nerve growth factor in oxidant-antioxidant balance and neuronal injury. II. A conditioning lesion paradigm. In: Journal of Neuroscience Research. 1990 ; Vol. 25, No. 3. pp. 369-374.
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