Role of peroxynitrite anion in renal hypothermic preservation injury

Martin J. Mangino, Mary S. Ametani, Barbara J. Gilligan, Csaba Szabo, Lionel Brounts, James H. Southard

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background. Peroxynitrite anions may play a role in normothermic renal ischemia and reperfusion. The purpose of this study was to determine if endogenous peroxynitrite anion is involved in renal preservation injury. Methods. Experiments were conducted in isolated canine renal tubules and in a canine autotransplant model of hypothermic preservation injury. Results. Isolated renal tubules demonstrated progressive loss of membrane transport function after reperfusion with increasing cold storage times in UW solution as assessed by tetraethylammonium cation transport (TEA). This transport defect was not altered by reperfusion in the presence of WW85, a peroxynitrite decomposition catalyst. Likewise, tubule LDH release was not altered by WW85. Renal tubules did not demonstrate any evidence of peroxynitrite formation after cold storage (0-120h) or after subsequent reperfusion in vitro as measured by nitrotyrosine adduct formation. Addition of exogenous peroxynitrite (1 mM) directly to freshly isolated renal tubules produced strong nitrotyrosine signals but failed to alter membrane function (TEA uptake). Conversely, SIN-1, a peroxynitrite generator molecule, failed to produce a nitrotyrosine signal in extracted renal tubule proteins but significantly impaired transport function. Finally, function of cold stored canine autografts was not affected by the scavenging of peroxynitrite anions (WW85) before kidney harvest and immediately at reperfusion. Tissue biopsies from cold stored kidney autografts also failed to show evidence of peroxynitrite synthesis either after cold storage (72 h) or after kidney transplantation (60 min reperfusion). Conclusions. This study concludes that peroxynitrite anions are not formed and are not involved in renal preservation injury.

Original languageEnglish (US)
Pages (from-to)1455-1460
Number of pages6
JournalTransplantation
Volume80
Issue number10
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Peroxynitrous Acid
Anions
Kidney
Wounds and Injuries
Reperfusion
Autografts
Canidae
Tetraethylammonium
Membranes
Kidney Transplantation
Cations
Ischemia
Biopsy

Keywords

  • Cold ischemia
  • Kidney transplantation
  • Nitric oxide
  • Reactive oxygen species
  • Superoxide

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Mangino, M. J., Ametani, M. S., Gilligan, B. J., Szabo, C., Brounts, L., & Southard, J. H. (2005). Role of peroxynitrite anion in renal hypothermic preservation injury. Transplantation, 80(10), 1455-1460. https://doi.org/10.1097/01.tp.0000181099.20099.d1

Role of peroxynitrite anion in renal hypothermic preservation injury. / Mangino, Martin J.; Ametani, Mary S.; Gilligan, Barbara J.; Szabo, Csaba; Brounts, Lionel; Southard, James H.

In: Transplantation, Vol. 80, No. 10, 11.2005, p. 1455-1460.

Research output: Contribution to journalArticle

Mangino, MJ, Ametani, MS, Gilligan, BJ, Szabo, C, Brounts, L & Southard, JH 2005, 'Role of peroxynitrite anion in renal hypothermic preservation injury', Transplantation, vol. 80, no. 10, pp. 1455-1460. https://doi.org/10.1097/01.tp.0000181099.20099.d1
Mangino MJ, Ametani MS, Gilligan BJ, Szabo C, Brounts L, Southard JH. Role of peroxynitrite anion in renal hypothermic preservation injury. Transplantation. 2005 Nov;80(10):1455-1460. https://doi.org/10.1097/01.tp.0000181099.20099.d1
Mangino, Martin J. ; Ametani, Mary S. ; Gilligan, Barbara J. ; Szabo, Csaba ; Brounts, Lionel ; Southard, James H. / Role of peroxynitrite anion in renal hypothermic preservation injury. In: Transplantation. 2005 ; Vol. 80, No. 10. pp. 1455-1460.
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KW - Cold ischemia

KW - Kidney transplantation

KW - Nitric oxide

KW - Reactive oxygen species

KW - Superoxide

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