Role of primary human alveolar epithelial cells in host defense against Francisella tularensis infection

Megan Gentry, Joanna Taormina, Richard Pyles, Linsey Yeager, Michelle Kirtley, Vsevolod Popov, Gary Klimpel, Tonyia Eaves-Pyles

Research output: Contribution to journalArticle

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Abstract

Francisella tularensis, an intracellular pathogen, is highly virulent when inhaled. Alveolar epithelial type I (ATI) and type II (ATII) cells line the majority of the alveolar surface and respond to inhaled pathogenic bacteria via cytokine secretion. We hypothesized that these cells contribute to the lung innate immune response to F. tularensis. Results demonstrated that the live vaccine strain (LVS) contacted ATI and ATII cells by 2 h following intranasal inoculation of mice. In culture, primary human ATI or ATII cells, grown on transwell filters, were stimulated on the apical (AP) surface with virulent F. tularensis Schu 4 or LVS. Basolateral (BL) conditioned medium (CM), collected 6 and 24 h later, was added to the BL surfaces of transwell cultures of primary human pulmonary microvasculature endothelial cells (HPMEC) prior to the addition of polymorphonuclear leukocytes (PMNs) or dendritic cells (DCs) to the AP surface. HPMEC responded to S4- or LVS-stimulated ATII, but not ATI, CM as evidenced by PMN and DC migration. Analysis of the AP and BL ATII CM revealed that both F. tularensis strains induced various levels of a variety of cytokines via NF-κB activation. ATII cells pretreated with an NF-κB inhibitor prior to F. tularensis stimulation substantially decreased interleukin-8 secretion, which did not occur through Toll-like receptor 2, 2/6, 4, or 5 stimulation. These data indicate a crucial role for ATII cells in the innate immune response to F. tularensis.

Original languageEnglish (US)
Pages (from-to)3969-3978
Number of pages10
JournalInfection and Immunity
Volume75
Issue number8
DOIs
StatePublished - Aug 2007

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Francisella tularensis
Tularemia
Alveolar Epithelial Cells
Conditioned Culture Medium
Vaccines
Microvessels
Innate Immunity
Lung
Dendritic Cells
Endothelial Cells
Cytokines
Toll-Like Receptor 2
Interleukin-8
Cell Movement
Neutrophils
Bacteria
Cell Line

ASJC Scopus subject areas

  • Immunology

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Role of primary human alveolar epithelial cells in host defense against Francisella tularensis infection. / Gentry, Megan; Taormina, Joanna; Pyles, Richard; Yeager, Linsey; Kirtley, Michelle; Popov, Vsevolod; Klimpel, Gary; Eaves-Pyles, Tonyia.

In: Infection and Immunity, Vol. 75, No. 8, 08.2007, p. 3969-3978.

Research output: Contribution to journalArticle

Gentry, Megan ; Taormina, Joanna ; Pyles, Richard ; Yeager, Linsey ; Kirtley, Michelle ; Popov, Vsevolod ; Klimpel, Gary ; Eaves-Pyles, Tonyia. / Role of primary human alveolar epithelial cells in host defense against Francisella tularensis infection. In: Infection and Immunity. 2007 ; Vol. 75, No. 8. pp. 3969-3978.
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