Role of protein kinase C and phosphorylation in bombesin-evoked gastrin release from isolated perfused rat stomach

Yan Shi Guo, James C. Thompson, Pomila Singh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have recently reported that bombesin (BBS)-stimulated gastrin release is principally dependent on a Ca2+/calmodulin intracellular pathway, and that it is independent of the cyclic AMP-mediated pathway. Recently it was demonstrated that stimulation of protein kinase C (PK-C) resulted in increased gastrin release from the isolated canine G-cells in cultures. The role of PK-C in the BBS-evoked gastrin release, however, remains unexamined. In this study we examined a possible role of PK-C in the secretion of BBS-stimulated gastrin from isolated perfused rat stomach. The effect of phosphorylation on gastrin release, in response to BBS, was also determined. Administration of phorbol ester (PMA 10-100 nM, a PK-C activator) alone significantly provoked gastrin release, but markedly inhibited the BBS (1 nM) stimulated gastrin secretion in a dose-dependent manner. Molybdic acid (phosphatase inhibitor), caused an enhancement of BBS-evoked gastrin response at doses of 5 or > 5 mM. These results suggest that: (1) diacylglycerol/PK-C pathway may exert a negative feedback control over BBS-induced gastrin release; (2) phosphorylation step is required for gastrin secretion in response to BBS.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalRegulatory Peptides
Volume26
Issue number1
DOIs
StatePublished - 1989

Fingerprint

Bombesin
Phosphorylation
Gastrins
Protein Kinase C
Rats
Stomach
Diacylglycerol Kinase
Gastrin-Secreting Cells
Diglycerides
Phorbol Esters
Calmodulin
Acid Phosphatase
Cyclic AMP
Feedback control
Canidae
Cell Culture Techniques
Feedback

Keywords

  • Bombesin
  • Isolated rat stomach
  • Phosphorylation
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Role of protein kinase C and phosphorylation in bombesin-evoked gastrin release from isolated perfused rat stomach. / Guo, Yan Shi; Thompson, James C.; Singh, Pomila.

In: Regulatory Peptides, Vol. 26, No. 1, 1989, p. 19-25.

Research output: Contribution to journalArticle

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