Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure

Krisztina Tar, Csilla Csortos, Istvan Czikora, Gabor Olah, Shwu Fan Ma, Raj Wadgaonkar, Pal Gergely, Joe G N Garcia, Alexander D. Verin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Our recently published data suggested the involvement of protein phosphatase 2A (PP2A) in endothelial cell (EC) barrier regulation (Tar et al. [2004] J Cell Biochem 92:534-546). In order to further elucidate the role of PP2A in the regulation of EC cytoskeleton and permeability, PP2A catalytic (PP2Ac) and A regulatory (PP2Aa)subunits were cloned and human pulmonary arterial EC (HPAEC) were transfected with PP2A mammalian expression constructs or infected with PP2A recombinant adenoviruses. Immunostaining of PP2Ac or of PP2Aa + c overexpressing HPAEC indicated actin cytoskeleton rearrangement. PP2A overexpression hindered or at least dramatically reduced thrombin- or nocodazole-induced F-actin stress fiber formation and microtubule (MT) dissolution. Accordingly, it also attenuated thrombin- or nocodazole-induced decrease in transendothelial electrical resistance indicative of barrier protection. Inhibition of PP2A by okadaic acid abolished its effect on agonist-induced changes in EC cytoskeleton; this indicates a critical role of PP2A activity in EC cytoskeletal maintenance. The overexpression of PP2A significantly attenuated thrombin- or nocodazole-induced phosphorylation of HSP27 and tau, two cytoskeletal proteins, which potentially could be involved in agonist-induced cytoskeletal rearrangement and in the increase of permeability. PP2A-mediated dephosphorylation of HSP27 and tau correlated with PP2A-induced preservation of EC cytoskeleton and barrier maintenance. Collectively, our observations clearly demonstrate the crucial role of PP2A in EC barrier protection.

Original languageEnglish (US)
Pages (from-to)931-953
Number of pages23
JournalJournal of Cellular Biochemistry
Volume98
Issue number4
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

Fingerprint

Protein Phosphatase 2
Endothelial cells
Cytoskeleton
Endothelial Cells
Nocodazole
Thrombin
Permeability
Actins
Maintenance
Okadaic Acid
Lung
Stress Fibers
Phosphorylation
Acoustic impedance
Cytoskeletal Proteins
Cytoprotection
Electric Impedance
Actin Cytoskeleton
Adenoviridae
Microtubules

Keywords

  • Endothelium
  • HSP27
  • Microfilaments
  • Microtubules
  • Permeability
  • Phosphatase 2A
  • Tau

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Tar, K., Csortos, C., Czikora, I., Olah, G., Ma, S. F., Wadgaonkar, R., ... Verin, A. D. (2006). Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure. Journal of Cellular Biochemistry, 98(4), 931-953. https://doi.org/10.1002/jcb.20829

Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure. / Tar, Krisztina; Csortos, Csilla; Czikora, Istvan; Olah, Gabor; Ma, Shwu Fan; Wadgaonkar, Raj; Gergely, Pal; Garcia, Joe G N; Verin, Alexander D.

In: Journal of Cellular Biochemistry, Vol. 98, No. 4, 01.07.2006, p. 931-953.

Research output: Contribution to journalArticle

Tar, K, Csortos, C, Czikora, I, Olah, G, Ma, SF, Wadgaonkar, R, Gergely, P, Garcia, JGN & Verin, AD 2006, 'Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure', Journal of Cellular Biochemistry, vol. 98, no. 4, pp. 931-953. https://doi.org/10.1002/jcb.20829
Tar, Krisztina ; Csortos, Csilla ; Czikora, Istvan ; Olah, Gabor ; Ma, Shwu Fan ; Wadgaonkar, Raj ; Gergely, Pal ; Garcia, Joe G N ; Verin, Alexander D. / Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure. In: Journal of Cellular Biochemistry. 2006 ; Vol. 98, No. 4. pp. 931-953.
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AU - Wadgaonkar, Raj

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