Role of rusticyanin in the electron transport process in Thiobacillus ferrooxidans.

Tapas Hazra, M. Mukherjea, R. N. Mukherjea

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Effect of diethyl dithiocarbamate (DEDC), an antimicrobial agent, on growth of Thiobacillus ferrooxidans, possibly by inhibiting rusticyanin present in the periplasmic space of the microorganism, has been studied to gain more insight into the electron transport chain in the bioleaching process. DEDC is found to form a stable complex with rusticyanin in solution and also in polyacrylamide gel. The spectrum of the complex is identical to that of Cu-DEDC complex, suggesting binding of DEDC with copper moiety of rusticyanin and resulting in inhibition of growth. In vitro reduction of purified rusticyanin by Fe(II) in absence of acid-stable cytochrome c is very slow, indicating the importance of cytochrome c in electron transport. Thus, in the iron oxidation process, acid-stable cytochrome c is the primary acceptor of electron, transferring the electron to rusticyanin at pH 2.0, which, in turn, affects electron transfer to iron-cytochrome c reductase around pH 5.5.

Original languageEnglish (US)
Pages (from-to)77-81
Number of pages5
JournalIndian Journal of Biochemistry and Biophysics
Volume29
Issue number1
StatePublished - Feb 1992
Externally publishedYes

Fingerprint

Thiobacillus
Electron Transport
Cytochromes c
Electrons
Iron
Cytochrome Reductases
Bioleaching
Periplasm
Acids
Growth
Anti-Infective Agents
Microorganisms
Copper
Oxidoreductases
rusticyanin
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Role of rusticyanin in the electron transport process in Thiobacillus ferrooxidans. / Hazra, Tapas; Mukherjea, M.; Mukherjea, R. N.

In: Indian Journal of Biochemistry and Biophysics, Vol. 29, No. 1, 02.1992, p. 77-81.

Research output: Contribution to journalArticle

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