Role of skeletal muscle in motor neuron development

Mark Baguma-Nibasheka, Anna Fracassi, Willard J. Costain, Sandra Moreno, Boris Kablar

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

The current paper is a continuation of our work most recently described in Kablar, 2011. Here, we show lists of up- and down-regulated genes obtained by a cDNA microarray analysis that compared developing mouse MyoD-/- limb musculature (MyoD-dependent, innervated by Lateral Motor Column motor neurons) and Myf5-/- back (epaxial) musculature (Myf5-dependent, innervated by Medial Motor Column motor neurons) to the control and to each other, at embryonic day 13.5 which coincides with the robust programmed cell death of motor neurons and the inability of myogenesis to undergo its normal progression in the absence of Myf5 and MyoD that at this embryonic day cannot substitute for each other. We wanted to see if/how the myogenic program couples with the neurotrophic one, and also to separate Lateral from Medial column trophic requirements, potentially relevant to Motor Neuron Diseases with the predilection for the Lateral column. Several follow-up steps revealed that Kif5c, Stxbp1 and Polb, differentially expressed in the MyoD-/- limb muscle, and Ppargc1a, Glrb and Hoxd10, differentially expressed in the Myf5-/- back muscle, are actually regulators of motor neuron numbers. We propose a series of follow-up experiments and various ways to consider our current data.

Original languageEnglish (US)
Pages (from-to)699-719
Number of pages21
JournalHistology and histopathology
Volume31
Issue number7
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Motor Neurons
Skeletal Muscle
Extremities
Back Muscles
Motor Neuron Disease
Muscle Development
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Cell Death
Muscles
Genes

Keywords

  • Microarray
  • Motor neuron
  • Myf5
  • MyoD
  • Myogenesis
  • Neurotrophins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Baguma-Nibasheka, M., Fracassi, A., Costain, W. J., Moreno, S., & Kablar, B. (2016). Role of skeletal muscle in motor neuron development. Histology and histopathology, 31(7), 699-719. https://doi.org/10.14670/HH-11-742

Role of skeletal muscle in motor neuron development. / Baguma-Nibasheka, Mark; Fracassi, Anna; Costain, Willard J.; Moreno, Sandra; Kablar, Boris.

In: Histology and histopathology, Vol. 31, No. 7, 01.01.2016, p. 699-719.

Research output: Contribution to journalReview article

Baguma-Nibasheka, M, Fracassi, A, Costain, WJ, Moreno, S & Kablar, B 2016, 'Role of skeletal muscle in motor neuron development', Histology and histopathology, vol. 31, no. 7, pp. 699-719. https://doi.org/10.14670/HH-11-742
Baguma-Nibasheka, Mark ; Fracassi, Anna ; Costain, Willard J. ; Moreno, Sandra ; Kablar, Boris. / Role of skeletal muscle in motor neuron development. In: Histology and histopathology. 2016 ; Vol. 31, No. 7. pp. 699-719.
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