Abstract
Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are members of the family of neurotrophins that are highly expressed in the adult hippocampus, and to a lesser extent, in the cerebral cortex and olfactory bulb. Since neuronal expression of neurotrophins is controlled by some neurotransmitters and there is a topographical correlation between neurotrophin expression and cholinergic terminal distribution from the cholinergic basal forebrain (CBF) neurons in these areas, the question arises as to whether the cholinergic system can also regulate neurotrophin gene expression in the CNS. When CBF neurons were selectively and completely destroyed by intraventricular injection of 192 IgG-saporin, resulting in a cholinergic deafferentation of the hippocampus, cortex, and olfactory bulb, there were no siginificant changes in NGF, BDNF and/or NT-3 mRNA levels in these areas from 1 week to 5 months after the lesion. These results suggest that afferents from CBF neurons may not play a significant role in maintaining basal levels of neurotrophin gene expression in the adult rat brain under physiological conditions. However, potential cholinergic regulation of brain neurontrophin expression may occur under other circumstances.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 247-254 |
| Number of pages | 8 |
| Journal | Brain Research |
| Volume | 705 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Dec 24 1995 |
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Keywords
- Cholinergic basal forebrain neuron
- Immunolesion
- mRNA expression
- Neurotrophin
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Role of the cholinergic system in the regulation of neurotrophin synthesis. / Yu, Juan; Pizzo, Donald P.; Hutton, Leslie A.; Perez-Polo, J. Regino.
In: Brain Research, Vol. 705, No. 1-2, 24.12.1995, p. 247-254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of the cholinergic system in the regulation of neurotrophin synthesis
AU - Yu, Juan
AU - Pizzo, Donald P.
AU - Hutton, Leslie A.
AU - Perez-Polo, J. Regino
PY - 1995/12/24
Y1 - 1995/12/24
N2 - Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are members of the family of neurotrophins that are highly expressed in the adult hippocampus, and to a lesser extent, in the cerebral cortex and olfactory bulb. Since neuronal expression of neurotrophins is controlled by some neurotransmitters and there is a topographical correlation between neurotrophin expression and cholinergic terminal distribution from the cholinergic basal forebrain (CBF) neurons in these areas, the question arises as to whether the cholinergic system can also regulate neurotrophin gene expression in the CNS. When CBF neurons were selectively and completely destroyed by intraventricular injection of 192 IgG-saporin, resulting in a cholinergic deafferentation of the hippocampus, cortex, and olfactory bulb, there were no siginificant changes in NGF, BDNF and/or NT-3 mRNA levels in these areas from 1 week to 5 months after the lesion. These results suggest that afferents from CBF neurons may not play a significant role in maintaining basal levels of neurotrophin gene expression in the adult rat brain under physiological conditions. However, potential cholinergic regulation of brain neurontrophin expression may occur under other circumstances.
AB - Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are members of the family of neurotrophins that are highly expressed in the adult hippocampus, and to a lesser extent, in the cerebral cortex and olfactory bulb. Since neuronal expression of neurotrophins is controlled by some neurotransmitters and there is a topographical correlation between neurotrophin expression and cholinergic terminal distribution from the cholinergic basal forebrain (CBF) neurons in these areas, the question arises as to whether the cholinergic system can also regulate neurotrophin gene expression in the CNS. When CBF neurons were selectively and completely destroyed by intraventricular injection of 192 IgG-saporin, resulting in a cholinergic deafferentation of the hippocampus, cortex, and olfactory bulb, there were no siginificant changes in NGF, BDNF and/or NT-3 mRNA levels in these areas from 1 week to 5 months after the lesion. These results suggest that afferents from CBF neurons may not play a significant role in maintaining basal levels of neurotrophin gene expression in the adult rat brain under physiological conditions. However, potential cholinergic regulation of brain neurontrophin expression may occur under other circumstances.
KW - Cholinergic basal forebrain neuron
KW - Immunolesion
KW - mRNA expression
KW - Neurotrophin
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UR - http://www.scopus.com/inward/citedby.url?scp=0029563699&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(95)01169-2
DO - 10.1016/0006-8993(95)01169-2
M3 - Article
C2 - 8821756
AN - SCOPUS:0029563699
VL - 705
SP - 247
EP - 254
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -