Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin

José M. Barral, Alex H. Hutagalung, Achim Brinker, F. Ulrich Hartl, Henry F. Epstein

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

The organization of myosin into motile cellular structures requires precise temporal and spatial regulation. Proteins containing a UCS (UNC-45/CRO1/She4p) domain are necessary for the incorporation of myosin into the contractile ring during cytokinesis and into thick filaments during muscle development. We report that the carboxyl-terminal regions of UNC-45 bound and exerted chaperone activity on the myosin head. The amino-terminal tetratricopeptide repeat domain of UNC-45 bound the molecular chaperone Hsp90. Thus, UNC-45 functions both as a molecular chaperone and as an Hsp90 co-chaperone for myosin, which can explain previous findings of altered assembly and decreased accumulation of myosin in UNC-45 mutants of Caenorhabditis elegans.

Original languageEnglish (US)
Pages (from-to)669-671
Number of pages3
JournalScience
Volume295
Issue number5555
DOIs
StatePublished - Jan 25 2002
Externally publishedYes

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Molecular Chaperones
Myosins
Proteins
Cytokinesis
Terminal Repeat Sequences
Muscle Development
Caenorhabditis elegans
Cellular Structures

ASJC Scopus subject areas

  • General

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Barral, J. M., Hutagalung, A. H., Brinker, A., Hartl, F. U., & Epstein, H. F. (2002). Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin. Science, 295(5555), 669-671. https://doi.org/10.1126/science.1066648

Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin. / Barral, José M.; Hutagalung, Alex H.; Brinker, Achim; Hartl, F. Ulrich; Epstein, Henry F.

In: Science, Vol. 295, No. 5555, 25.01.2002, p. 669-671.

Research output: Contribution to journalArticle

Barral, JM, Hutagalung, AH, Brinker, A, Hartl, FU & Epstein, HF 2002, 'Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin', Science, vol. 295, no. 5555, pp. 669-671. https://doi.org/10.1126/science.1066648
Barral JM, Hutagalung AH, Brinker A, Hartl FU, Epstein HF. Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin. Science. 2002 Jan 25;295(5555):669-671. https://doi.org/10.1126/science.1066648
Barral, José M. ; Hutagalung, Alex H. ; Brinker, Achim ; Hartl, F. Ulrich ; Epstein, Henry F. / Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin. In: Science. 2002 ; Vol. 295, No. 5555. pp. 669-671.
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