TY - JOUR
T1 - Role of the suppressor of cytokine signaling 2 (SOCS2) during meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5)
AU - Aparecida Silva Barbosa, Aline
AU - Freitas Versiani, Alice
AU - Fonseca da Cunha Sousa, Larissa
AU - Silva de Miranda, Aline
AU - Gasparini, Marcela Ribeiro
AU - Brant, Fátima
AU - Silva, Daniele Gonçalves
AU - Luisa Quintino-de-Carvalho, Iracema
AU - Marianetti Soriani, Frederico
AU - Guimarães da Fonseca, Flávio
AU - César Vasconcelos, Anilton
AU - da Silva Barcelos, Lucíola
AU - Martins Teixeira, Mauro
AU - Lúcio Teixeira, Antônio
AU - Machado, Fabiana Simão
AU - Barbosa-Stancioli, Edel Figueiredo
AU - Rachid, Milene Alvarenga
N1 - Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The role of suppressors of cytokine signaling (SOCS) in meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) was evaluated by intracranial infection in C57BL/6 wild-type mice (WT) and SOCS2 deficient mice (SOCS2-/-). Both infected groups presented weight loss, ruffled fur and hunched posture. Additionally, infected SOCS2-/- mice showed swollen chamfer and progressive depression. Infected WT animals developed mild meningitis, characterized by infiltration of mononuclear cells. Moreover, viral DNA was detected in liver and lung from infected WT group. This group also showed elevated brain levels of IFN-γ, IL-10, CXCL1 and CCL5, when compared with non-infected WT animals. Brain inflammation was exacerbated in infected SOCS2-/- mice with widespread distribution of the virus and increased brain levels of TNF-α, IFN-γ, IL-10, IL-12, CXCL1 and CCL5, when compared with WT infected mice. Moreover, infected SOCS2 deficient mice exhibited reduced brain mRNA expression of IFNα and IFNβ and increased expression of mRNA of SOCS1, compared with infected WT mice. Taken together, our study provides an insight into the role of SOCS2 in modulating the immune response to BoHV-5 infection.
AB - The role of suppressors of cytokine signaling (SOCS) in meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) was evaluated by intracranial infection in C57BL/6 wild-type mice (WT) and SOCS2 deficient mice (SOCS2-/-). Both infected groups presented weight loss, ruffled fur and hunched posture. Additionally, infected SOCS2-/- mice showed swollen chamfer and progressive depression. Infected WT animals developed mild meningitis, characterized by infiltration of mononuclear cells. Moreover, viral DNA was detected in liver and lung from infected WT group. This group also showed elevated brain levels of IFN-γ, IL-10, CXCL1 and CCL5, when compared with non-infected WT animals. Brain inflammation was exacerbated in infected SOCS2-/- mice with widespread distribution of the virus and increased brain levels of TNF-α, IFN-γ, IL-10, IL-12, CXCL1 and CCL5, when compared with WT infected mice. Moreover, infected SOCS2 deficient mice exhibited reduced brain mRNA expression of IFNα and IFNβ and increased expression of mRNA of SOCS1, compared with infected WT mice. Taken together, our study provides an insight into the role of SOCS2 in modulating the immune response to BoHV-5 infection.
KW - Bovine herpesvirus 5
KW - Brain
KW - Inflammation
KW - Mice
KW - SOCS2
UR - http://www.scopus.com/inward/record.url?scp=84973102966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973102966&partnerID=8YFLogxK
U2 - 10.1016/j.cimid.2016.05.003
DO - 10.1016/j.cimid.2016.05.003
M3 - Article
C2 - 27477504
AN - SCOPUS:84973102966
SN - 0147-9571
VL - 47
SP - 26
EP - 31
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
ER -