Role of TNF-α in gut mucosal changes after severe burn

Marcus Spies, Vicky L. Chappell, Mohan R. Dasu, David Herndon, James C. Thompson, Steven Wolf, Texas

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53 Scopus citations

Abstract

Gut epithelial cell death by apoptosis is increased in the gut epithelium after severe burn associated with mucosal atrophy. We hypothesized that tumor necrosis factor (TNF)-α-TNF receptor (TNFR) interaction activates apoptosis in small bowel mucosal cells after severe burn. C57BL6 mice received a 30% total body surface area scald burn and were treated with neutralizing anti-TNF-α. The proximal small bowel was assessed for mucosal atrophy. Proliferation and apoptosis of mucosal cells were assessed by proliferative cell nuclear antigen-immunostaining and terminal deoxyuridine nick-end labeling assay, respectively. Mucosal height and mucosal cell number decreased after burn. Anti-TNF-a-treated mice showed significantly less mucosal atrophy. Proliferation of intestinal cells was not changed with burn or anti-TNF-α treatment. An over threefold increase in apoptotic cell number was seen after burn, which was diminished by anti-TNF-α treatment. Changes in gut mucosal homeostasis after severe burn are affected, in part, by the activation of apoptosis by TNF-α-TNFR interaction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number3 46-3
StatePublished - Sep 2002

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Keywords

  • Apoptosis
  • Mucosal atrophy
  • Proliferation
  • Small bowel mucosa
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Spies, M., Chappell, V. L., Dasu, M. R., Herndon, D., Thompson, J. C., Wolf, S., & Texas (2002). Role of TNF-α in gut mucosal changes after severe burn. American Journal of Physiology - Gastrointestinal and Liver Physiology, 283(3 46-3).