Role of TNF-α in gut mucosal changes after severe burn

Marcus Spies, Vicky L. Chappell, Mohan R. Dasu, David Herndon, James C. Thompson, Steven Wolf, Texas

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Gut epithelial cell death by apoptosis is increased in the gut epithelium after severe burn associated with mucosal atrophy. We hypothesized that tumor necrosis factor (TNF)-α-TNF receptor (TNFR) interaction activates apoptosis in small bowel mucosal cells after severe burn. C57BL6 mice received a 30% total body surface area scald burn and were treated with neutralizing anti-TNF-α. The proximal small bowel was assessed for mucosal atrophy. Proliferation and apoptosis of mucosal cells were assessed by proliferative cell nuclear antigen-immunostaining and terminal deoxyuridine nick-end labeling assay, respectively. Mucosal height and mucosal cell number decreased after burn. Anti-TNF-a-treated mice showed significantly less mucosal atrophy. Proliferation of intestinal cells was not changed with burn or anti-TNF-α treatment. An over threefold increase in apoptotic cell number was seen after burn, which was diminished by anti-TNF-α treatment. Changes in gut mucosal homeostasis after severe burn are affected, in part, by the activation of apoptosis by TNF-α-TNFR interaction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number3 46-3
StatePublished - Sep 2002

Fingerprint

Tumor Necrosis Factor-alpha
Tumor Necrosis Factor Receptors
Apoptosis
Atrophy
Cell Count
Nuclear Antigens
Deoxyuridine
Body Surface Area
Homeostasis
Cell Death
Epithelium
Epithelial Cells
Cell Proliferation

Keywords

  • Apoptosis
  • Mucosal atrophy
  • Proliferation
  • Small bowel mucosa
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Spies, M., Chappell, V. L., Dasu, M. R., Herndon, D., Thompson, J. C., Wolf, S., & Texas (2002). Role of TNF-α in gut mucosal changes after severe burn. American Journal of Physiology - Gastrointestinal and Liver Physiology, 283(3 46-3).

Role of TNF-α in gut mucosal changes after severe burn. / Spies, Marcus; Chappell, Vicky L.; Dasu, Mohan R.; Herndon, David; Thompson, James C.; Wolf, Steven; Texas.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 3 46-3, 09.2002.

Research output: Contribution to journalArticle

Spies, M, Chappell, VL, Dasu, MR, Herndon, D, Thompson, JC, Wolf, S & Texas 2002, 'Role of TNF-α in gut mucosal changes after severe burn', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 283, no. 3 46-3.
Spies, Marcus ; Chappell, Vicky L. ; Dasu, Mohan R. ; Herndon, David ; Thompson, James C. ; Wolf, Steven ; Texas. / Role of TNF-α in gut mucosal changes after severe burn. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 3 46-3.
@article{a5a1b208f2564ffaa648da8ce1128b17,
title = "Role of TNF-α in gut mucosal changes after severe burn",
abstract = "Gut epithelial cell death by apoptosis is increased in the gut epithelium after severe burn associated with mucosal atrophy. We hypothesized that tumor necrosis factor (TNF)-α-TNF receptor (TNFR) interaction activates apoptosis in small bowel mucosal cells after severe burn. C57BL6 mice received a 30{\%} total body surface area scald burn and were treated with neutralizing anti-TNF-α. The proximal small bowel was assessed for mucosal atrophy. Proliferation and apoptosis of mucosal cells were assessed by proliferative cell nuclear antigen-immunostaining and terminal deoxyuridine nick-end labeling assay, respectively. Mucosal height and mucosal cell number decreased after burn. Anti-TNF-a-treated mice showed significantly less mucosal atrophy. Proliferation of intestinal cells was not changed with burn or anti-TNF-α treatment. An over threefold increase in apoptotic cell number was seen after burn, which was diminished by anti-TNF-α treatment. Changes in gut mucosal homeostasis after severe burn are affected, in part, by the activation of apoptosis by TNF-α-TNFR interaction.",
keywords = "Apoptosis, Mucosal atrophy, Proliferation, Small bowel mucosa, Tumor necrosis factor-α",
author = "Marcus Spies and Chappell, {Vicky L.} and Dasu, {Mohan R.} and David Herndon and Thompson, {James C.} and Steven Wolf and Texas",
year = "2002",
month = "9",
language = "English (US)",
volume = "283",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "3 46-3",

}

TY - JOUR

T1 - Role of TNF-α in gut mucosal changes after severe burn

AU - Spies, Marcus

AU - Chappell, Vicky L.

AU - Dasu, Mohan R.

AU - Herndon, David

AU - Thompson, James C.

AU - Wolf, Steven

AU - Texas,

PY - 2002/9

Y1 - 2002/9

N2 - Gut epithelial cell death by apoptosis is increased in the gut epithelium after severe burn associated with mucosal atrophy. We hypothesized that tumor necrosis factor (TNF)-α-TNF receptor (TNFR) interaction activates apoptosis in small bowel mucosal cells after severe burn. C57BL6 mice received a 30% total body surface area scald burn and were treated with neutralizing anti-TNF-α. The proximal small bowel was assessed for mucosal atrophy. Proliferation and apoptosis of mucosal cells were assessed by proliferative cell nuclear antigen-immunostaining and terminal deoxyuridine nick-end labeling assay, respectively. Mucosal height and mucosal cell number decreased after burn. Anti-TNF-a-treated mice showed significantly less mucosal atrophy. Proliferation of intestinal cells was not changed with burn or anti-TNF-α treatment. An over threefold increase in apoptotic cell number was seen after burn, which was diminished by anti-TNF-α treatment. Changes in gut mucosal homeostasis after severe burn are affected, in part, by the activation of apoptosis by TNF-α-TNFR interaction.

AB - Gut epithelial cell death by apoptosis is increased in the gut epithelium after severe burn associated with mucosal atrophy. We hypothesized that tumor necrosis factor (TNF)-α-TNF receptor (TNFR) interaction activates apoptosis in small bowel mucosal cells after severe burn. C57BL6 mice received a 30% total body surface area scald burn and were treated with neutralizing anti-TNF-α. The proximal small bowel was assessed for mucosal atrophy. Proliferation and apoptosis of mucosal cells were assessed by proliferative cell nuclear antigen-immunostaining and terminal deoxyuridine nick-end labeling assay, respectively. Mucosal height and mucosal cell number decreased after burn. Anti-TNF-a-treated mice showed significantly less mucosal atrophy. Proliferation of intestinal cells was not changed with burn or anti-TNF-α treatment. An over threefold increase in apoptotic cell number was seen after burn, which was diminished by anti-TNF-α treatment. Changes in gut mucosal homeostasis after severe burn are affected, in part, by the activation of apoptosis by TNF-α-TNFR interaction.

KW - Apoptosis

KW - Mucosal atrophy

KW - Proliferation

KW - Small bowel mucosa

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=0036720894&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036720894&partnerID=8YFLogxK

M3 - Article

C2 - 12181186

AN - SCOPUS:0036720894

VL - 283

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 3 46-3

ER -