Role of tumor necrosis factor-α in the premature rupture of membranes and preterm labor pathways

Stephen J. Fortunato, Ramkumar Menon, Salvatore J. Lombardi

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

OBJECTIVE: To further delineate the differences between the preterm labor and premature rupture of the membrane pathways, we investigated the role of the inflammatory cytokines as activators of matrix metalloproteinases 2 and 9 in human fetal membranes. STUDY DESIGN: Normal amniochorionic membrane that is maintained in an organ explant system was stimulated with interleukin-1β, tumor necrosis factor-α, or interleukin-6. The expression and activity of matrix metalloproteinases 2 and 9 in amniochorion was documented with reverse transcriptase-polymerase chain reaction and specific substrate activity assays. The matrix metalloproteinase inhibitor, tissue inhibitor of metalloproteinase-1, concentration was measured by enzyme-linked immunosorbent assay. RESULTS: Interleukin-1β, tumor necrosis factor-α, and interleukin-6 induced the expression of matrix metalloproteinase-9 messenger RNA, whereas matrix metalloproteinase-2 expression was constitutive in control and cytokine-stimulated tissues. Matrix metalloproteinase-2 activity did not change after cytokine stimulation. Active matrix metalloproteinase-9 was significantly higher in tumor necrosis factor-stimulated tissues, which conversely were not changed after interleukin-1 or interleukin-6 stimulation. Tissue inhibitor of metalloproteinase-1 levels were decreased after interleukin-1 and tumor necrosis factor stimulation but changed after interleukin-6 stimulation. CONCLUSION: Only tumor necrosis factor-α increases matrix metalloproteinase-9 activity in amniochorion.

Original languageEnglish (US)
Pages (from-to)1159-1162
Number of pages4
JournalAmerican Journal of Obstetrics and Gynecology
Volume187
Issue number5
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Premature Obstetric Labor
Matrix Metalloproteinase 9
Matrix Metalloproteinase 2
Rupture
Interleukin-1
Tumor Necrosis Factor-alpha
Interleukin-6
Membranes
Tissue Inhibitor of Metalloproteinase-1
Cytokines
Extraembryonic Membranes
Matrix Metalloproteinase Inhibitors
Reverse Transcriptase Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
Messenger RNA

Keywords

  • Amniochorion
  • Cytokine
  • Matrix metalloproteinase
  • Premature rupture of membranes
  • Preterm labor

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Role of tumor necrosis factor-α in the premature rupture of membranes and preterm labor pathways. / Fortunato, Stephen J.; Menon, Ramkumar; Lombardi, Salvatore J.

In: American Journal of Obstetrics and Gynecology, Vol. 187, No. 5, 01.11.2002, p. 1159-1162.

Research output: Contribution to journalArticle

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