ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation

Istvan Boldogh, Attila Bacsi, Barun Choudhury, Nilesh Dharajiya, Rafeul Alam, Tapas Hazra, Sankar Mitra, Randall M. Goldblum, Sanjiv Sur

Research output: Contribution to journalArticle

256 Citations (Scopus)

Abstract

Pollen exposure induces allergic airway inflammation in sensitized subjects. The role of antigenic pollen proteins in the induction of allergic airway inflammation is well characterized, but the contribution of other constituents in pollen grains to this process is unknown. Here we show that pollen grains and their extracts contain intrinsic NADPH oxidases. The pollen NADPH oxidases rapidly increased the levels of ROS in lung epithelium as well as the amount of oxidized glutathione (GSSG) and 4-hydroxynonenal (4-HNE) in airway-lining fluid. These oxidases, as well as products of oxidative stress (such as GSSG and 4-HNE) generated by these enzymes, induced neutrophil recruitment to the airways independent of the adaptive immune response. Removal of pollen NADPH oxidase activity from the challenge material reduced antigen-induced allergic airway inflammation, the number of mucin-containing cells in airway epithelium, and antigen-specific IgE levels in sensitized mice. Furthermore, challenge with Amb a 1, the major antigen in ragweed pollen extract that does not possess NADPH oxidase activity, induced low-grade allergic airway inflammation. Addition of GSSG or 4-HNE to Amb a 1 challenge material boosted allergic airway inflammation. We propose that oxidative stress generated by pollen NADPH oxidases (signal 1) augments allergic airway inflammation induced by pollen antigen (signal 2).

Original languageEnglish (US)
Pages (from-to)2169-2179
Number of pages11
JournalJournal of Clinical Investigation
Volume115
Issue number8
DOIs
StatePublished - Aug 2005

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NADPH Oxidase
Pollen
Inflammation
Antigens
Glutathione Disulfide
Oxidative Stress
Epithelium
Neutrophil Infiltration
Adaptive Immunity
Mucins
Immunoglobulin E
Oxidoreductases
Lung
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

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ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation. / Boldogh, Istvan; Bacsi, Attila; Choudhury, Barun; Dharajiya, Nilesh; Alam, Rafeul; Hazra, Tapas; Mitra, Sankar; Goldblum, Randall M.; Sur, Sanjiv.

In: Journal of Clinical Investigation, Vol. 115, No. 8, 08.2005, p. 2169-2179.

Research output: Contribution to journalArticle

Boldogh, Istvan ; Bacsi, Attila ; Choudhury, Barun ; Dharajiya, Nilesh ; Alam, Rafeul ; Hazra, Tapas ; Mitra, Sankar ; Goldblum, Randall M. ; Sur, Sanjiv. / ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation. In: Journal of Clinical Investigation. 2005 ; Vol. 115, No. 8. pp. 2169-2179.
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