RSV-induced prostaglandin E2 production occurs via cPLA2 activation

Role in viral replication

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Prostaglandins (PGs) are lipid mediators that participate in the regulation of immunological and inflammatory responses, and PG production can affect viral replication. In this study, we have investigated the mechanism of PGE2 production in airway epithelial cells, following respiratory syncytial virus (RSV) infection, and its role in viral replication. We show that RSV infection strongly induces PGE2 secretion, in a time- and replication-dependent manner, through increased cyclooxygenase-2 (COX-2) expression, which occurs independently from viral or cellular protein synthesis. RSV infection induces arachidonic acid release through induction of cytoplasmic phospholipase A 2 (cPLA2) enzymatic activity and its membrane translocation. Specific inhibitors of cPLA2 significantly block RSV-induced PGE2 secretion, indicating a key role of cPLA2 in viral-induced PG production. Blocking PG secretion, through cPLA2 or COX-2 inhibition, results in impairment of RSV replication and subsequent RSV-mediated epithelial cell responses, suggesting that inhibition of PG secretion could be beneficial in RSV infection by reducing proinflammatory mediator production.

Original languageEnglish (US)
Pages (from-to)12-24
Number of pages13
JournalVirology
Volume343
Issue number1
DOIs
StatePublished - Dec 5 2005

Fingerprint

Respiratory Syncytial Viruses
Phospholipases A
Respiratory Syncytial Virus Infections
Dinoprostone
Prostaglandins
Cyclooxygenase 2
Epithelial Cells
Virus Replication
Arachidonic Acid
Lipids
Membranes
Proteins

Keywords

  • Airway epithelial cells
  • Inflammation
  • Prostaglandin
  • RSV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

RSV-induced prostaglandin E2 production occurs via cPLA2 activation : Role in viral replication. / Liu, Tianshuang; Zaman, Wahiduz; Kaphalia, Bhupendra; Ansari, Ghulam; Garofalo, Roberto; Casola, Antonella.

In: Virology, Vol. 343, No. 1, 05.12.2005, p. 12-24.

Research output: Contribution to journalArticle

@article{1371b45c817d4df295e383f9832c18e7,
title = "RSV-induced prostaglandin E2 production occurs via cPLA2 activation: Role in viral replication",
abstract = "Prostaglandins (PGs) are lipid mediators that participate in the regulation of immunological and inflammatory responses, and PG production can affect viral replication. In this study, we have investigated the mechanism of PGE2 production in airway epithelial cells, following respiratory syncytial virus (RSV) infection, and its role in viral replication. We show that RSV infection strongly induces PGE2 secretion, in a time- and replication-dependent manner, through increased cyclooxygenase-2 (COX-2) expression, which occurs independently from viral or cellular protein synthesis. RSV infection induces arachidonic acid release through induction of cytoplasmic phospholipase A 2 (cPLA2) enzymatic activity and its membrane translocation. Specific inhibitors of cPLA2 significantly block RSV-induced PGE2 secretion, indicating a key role of cPLA2 in viral-induced PG production. Blocking PG secretion, through cPLA2 or COX-2 inhibition, results in impairment of RSV replication and subsequent RSV-mediated epithelial cell responses, suggesting that inhibition of PG secretion could be beneficial in RSV infection by reducing proinflammatory mediator production.",
keywords = "Airway epithelial cells, Inflammation, Prostaglandin, RSV",
author = "Tianshuang Liu and Wahiduz Zaman and Bhupendra Kaphalia and Ghulam Ansari and Roberto Garofalo and Antonella Casola",
year = "2005",
month = "12",
day = "5",
doi = "10.1016/j.virol.2005.08.012",
language = "English (US)",
volume = "343",
pages = "12--24",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - RSV-induced prostaglandin E2 production occurs via cPLA2 activation

T2 - Role in viral replication

AU - Liu, Tianshuang

AU - Zaman, Wahiduz

AU - Kaphalia, Bhupendra

AU - Ansari, Ghulam

AU - Garofalo, Roberto

AU - Casola, Antonella

PY - 2005/12/5

Y1 - 2005/12/5

N2 - Prostaglandins (PGs) are lipid mediators that participate in the regulation of immunological and inflammatory responses, and PG production can affect viral replication. In this study, we have investigated the mechanism of PGE2 production in airway epithelial cells, following respiratory syncytial virus (RSV) infection, and its role in viral replication. We show that RSV infection strongly induces PGE2 secretion, in a time- and replication-dependent manner, through increased cyclooxygenase-2 (COX-2) expression, which occurs independently from viral or cellular protein synthesis. RSV infection induces arachidonic acid release through induction of cytoplasmic phospholipase A 2 (cPLA2) enzymatic activity and its membrane translocation. Specific inhibitors of cPLA2 significantly block RSV-induced PGE2 secretion, indicating a key role of cPLA2 in viral-induced PG production. Blocking PG secretion, through cPLA2 or COX-2 inhibition, results in impairment of RSV replication and subsequent RSV-mediated epithelial cell responses, suggesting that inhibition of PG secretion could be beneficial in RSV infection by reducing proinflammatory mediator production.

AB - Prostaglandins (PGs) are lipid mediators that participate in the regulation of immunological and inflammatory responses, and PG production can affect viral replication. In this study, we have investigated the mechanism of PGE2 production in airway epithelial cells, following respiratory syncytial virus (RSV) infection, and its role in viral replication. We show that RSV infection strongly induces PGE2 secretion, in a time- and replication-dependent manner, through increased cyclooxygenase-2 (COX-2) expression, which occurs independently from viral or cellular protein synthesis. RSV infection induces arachidonic acid release through induction of cytoplasmic phospholipase A 2 (cPLA2) enzymatic activity and its membrane translocation. Specific inhibitors of cPLA2 significantly block RSV-induced PGE2 secretion, indicating a key role of cPLA2 in viral-induced PG production. Blocking PG secretion, through cPLA2 or COX-2 inhibition, results in impairment of RSV replication and subsequent RSV-mediated epithelial cell responses, suggesting that inhibition of PG secretion could be beneficial in RSV infection by reducing proinflammatory mediator production.

KW - Airway epithelial cells

KW - Inflammation

KW - Prostaglandin

KW - RSV

UR - http://www.scopus.com/inward/record.url?scp=27644566970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27644566970&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2005.08.012

DO - 10.1016/j.virol.2005.08.012

M3 - Article

VL - 343

SP - 12

EP - 24

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -