Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication

Junhong Han, Hui Zhou, Bruce Horazdovsky, Kangling Zhang, Rui Ming Xu, Zhiguo Zhang

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.

Original languageEnglish (US)
Pages (from-to)653-655
Number of pages3
JournalScience
Volume315
Issue number5812
DOIs
StatePublished - Feb 2 2007
Externally publishedYes

Fingerprint

Acetylation
DNA Replication
Histone Acetyltransferases
Histones
Lysine
Genes
Chromosome Breakage
Sequence Homology
S Phase
Aspartic Acid
DNA

ASJC Scopus subject areas

  • General

Cite this

Han, J., Zhou, H., Horazdovsky, B., Zhang, K., Xu, R. M., & Zhang, Z. (2007). Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication. Science, 315(5812), 653-655. https://doi.org/10.1126/science.1133234

Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication. / Han, Junhong; Zhou, Hui; Horazdovsky, Bruce; Zhang, Kangling; Xu, Rui Ming; Zhang, Zhiguo.

In: Science, Vol. 315, No. 5812, 02.02.2007, p. 653-655.

Research output: Contribution to journalArticle

Han, J, Zhou, H, Horazdovsky, B, Zhang, K, Xu, RM & Zhang, Z 2007, 'Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication', Science, vol. 315, no. 5812, pp. 653-655. https://doi.org/10.1126/science.1133234
Han, Junhong ; Zhou, Hui ; Horazdovsky, Bruce ; Zhang, Kangling ; Xu, Rui Ming ; Zhang, Zhiguo. / Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication. In: Science. 2007 ; Vol. 315, No. 5812. pp. 653-655.
@article{026a5b045e5342a98fff3f43b7982b3a,
title = "Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication",
abstract = "Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.",
author = "Junhong Han and Hui Zhou and Bruce Horazdovsky and Kangling Zhang and Xu, {Rui Ming} and Zhiguo Zhang",
year = "2007",
month = "2",
day = "2",
doi = "10.1126/science.1133234",
language = "English (US)",
volume = "315",
pages = "653--655",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5812",

}

TY - JOUR

T1 - Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication

AU - Han, Junhong

AU - Zhou, Hui

AU - Horazdovsky, Bruce

AU - Zhang, Kangling

AU - Xu, Rui Ming

AU - Zhang, Zhiguo

PY - 2007/2/2

Y1 - 2007/2/2

N2 - Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.

AB - Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.

UR - http://www.scopus.com/inward/record.url?scp=33846796258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846796258&partnerID=8YFLogxK

U2 - 10.1126/science.1133234

DO - 10.1126/science.1133234

M3 - Article

C2 - 17272723

AN - SCOPUS:33846796258

VL - 315

SP - 653

EP - 655

JO - Science

JF - Science

SN - 0036-8075

IS - 5812

ER -