S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs

Elena Sbrana, Emilia Bramanti, Maria C. Spinetti, Giorgio Raspi

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.

Original languageEnglish (US)
Pages (from-to)1518-1521
Number of pages4
JournalELECTROPHORESIS
Volume25
Issue number10-11
StatePublished - Jun 1 2004

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Keywords

  • Adenosine analogs
  • Antiviral drugs
  • Ebola
  • S-Adenosyl-L-homocysteine hydrolase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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