S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs

Elena Sbrana, Emilia Bramanti, Maria C. Spinetti, Giorgio Raspi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.

Original languageEnglish (US)
Pages (from-to)1518-1521
Number of pages4
JournalElectrophoresis
Volume25
Issue number10-11
StatePublished - Jun 2004

Fingerprint

S-Adenosylmethionine
Capillary electrophoresis
Capillary Electrophoresis
Homocysteine
Adenosine
Antiviral Agents
Monitoring
Lasers
Plasma (human)
Investigational Therapies
Hydrolases
Fluorescence
Clinical Trials
Testing
Infection

Keywords

  • Adenosine analogs
  • Antiviral drugs
  • Ebola
  • S-Adenosyl-L-homocysteine hydrolase

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs. / Sbrana, Elena; Bramanti, Emilia; Spinetti, Maria C.; Raspi, Giorgio.

In: Electrophoresis, Vol. 25, No. 10-11, 06.2004, p. 1518-1521.

Research output: Contribution to journalArticle

@article{b95c6e09bd95404bbe38355087480a35,
title = "S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs",
abstract = "S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.",
keywords = "Adenosine analogs, Antiviral drugs, Ebola, S-Adenosyl-L-homocysteine hydrolase",
author = "Elena Sbrana and Emilia Bramanti and Spinetti, {Maria C.} and Giorgio Raspi",
year = "2004",
month = "6",
language = "English (US)",
volume = "25",
pages = "1518--1521",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "10-11",

}

TY - JOUR

T1 - S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs

AU - Sbrana, Elena

AU - Bramanti, Emilia

AU - Spinetti, Maria C.

AU - Raspi, Giorgio

PY - 2004/6

Y1 - 2004/6

N2 - S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.

AB - S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.

KW - Adenosine analogs

KW - Antiviral drugs

KW - Ebola

KW - S-Adenosyl-L-homocysteine hydrolase

UR - http://www.scopus.com/inward/record.url?scp=3042774143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042774143&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 1518

EP - 1521

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 10-11

ER -