Abstract
Genetically modified crops have resistance to abiotic stress by introduction of choline oxidase protein. In the present study, the safety of choline oxidase protein derived from Arthrobacter globiformis was assessed for toxicity and allergenicity. The protein was stable at 90 ° C for 1 h. Toxicity studies of choline oxidase in mice showed no significant difference (p > 0.05) from control in terms of growth, body weight, food consumption, and blood biochemical indices. Histology of gut tissue of mice fed protein showed normal gastric mucosal lining and villi in jejunum and ileum sections. Specific IgE in serum and IL-4 release in splenic culture supernatant were low in choline oxidase treated mice, comparable to control. Intravenous challenge with choline oxidase did not induce any adverse reaction, unlike ovalbumin group mice. Histology of lung tissues from choline oxidase sensitized mice showed normal airways, whereas ovalbumin-sensitized mice showed inflammed airways with eosinophilic infiltration and bronchoconstriction. ELISA carried out with food allergic patients' sera revealed no significant IgE affinity with choline oxidase. Also, choline oxidase did not show any symptoms of toxicity and allergenicity in mice.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12099-12104 |
| Number of pages | 6 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 56 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 24 2008 |
| Externally published | Yes |
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Keywords
- Allergenicity
- Arthrobacter globiformis
- Choline oxidase
- GM plants
- Toxicity
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
Cite this
Safety assessment of bacterial choline oxidase protein introduced in transgenic crops for tolerance against abiotic stress. / Singh, Abinav K.; Singh, Bhanu P.; Prasad, G. B K S; Gaur, Shailendra N.; Arora, Naveen.
In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 24, 24.12.2008, p. 12099-12104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Safety assessment of bacterial choline oxidase protein introduced in transgenic crops for tolerance against abiotic stress
AU - Singh, Abinav K.
AU - Singh, Bhanu P.
AU - Prasad, G. B K S
AU - Gaur, Shailendra N.
AU - Arora, Naveen
PY - 2008/12/24
Y1 - 2008/12/24
N2 - Genetically modified crops have resistance to abiotic stress by introduction of choline oxidase protein. In the present study, the safety of choline oxidase protein derived from Arthrobacter globiformis was assessed for toxicity and allergenicity. The protein was stable at 90 ° C for 1 h. Toxicity studies of choline oxidase in mice showed no significant difference (p > 0.05) from control in terms of growth, body weight, food consumption, and blood biochemical indices. Histology of gut tissue of mice fed protein showed normal gastric mucosal lining and villi in jejunum and ileum sections. Specific IgE in serum and IL-4 release in splenic culture supernatant were low in choline oxidase treated mice, comparable to control. Intravenous challenge with choline oxidase did not induce any adverse reaction, unlike ovalbumin group mice. Histology of lung tissues from choline oxidase sensitized mice showed normal airways, whereas ovalbumin-sensitized mice showed inflammed airways with eosinophilic infiltration and bronchoconstriction. ELISA carried out with food allergic patients' sera revealed no significant IgE affinity with choline oxidase. Also, choline oxidase did not show any symptoms of toxicity and allergenicity in mice.
AB - Genetically modified crops have resistance to abiotic stress by introduction of choline oxidase protein. In the present study, the safety of choline oxidase protein derived from Arthrobacter globiformis was assessed for toxicity and allergenicity. The protein was stable at 90 ° C for 1 h. Toxicity studies of choline oxidase in mice showed no significant difference (p > 0.05) from control in terms of growth, body weight, food consumption, and blood biochemical indices. Histology of gut tissue of mice fed protein showed normal gastric mucosal lining and villi in jejunum and ileum sections. Specific IgE in serum and IL-4 release in splenic culture supernatant were low in choline oxidase treated mice, comparable to control. Intravenous challenge with choline oxidase did not induce any adverse reaction, unlike ovalbumin group mice. Histology of lung tissues from choline oxidase sensitized mice showed normal airways, whereas ovalbumin-sensitized mice showed inflammed airways with eosinophilic infiltration and bronchoconstriction. ELISA carried out with food allergic patients' sera revealed no significant IgE affinity with choline oxidase. Also, choline oxidase did not show any symptoms of toxicity and allergenicity in mice.
KW - Allergenicity
KW - Arthrobacter globiformis
KW - Choline oxidase
KW - GM plants
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=58849123376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58849123376&partnerID=8YFLogxK
U2 - 10.1021/jf8027073
DO - 10.1021/jf8027073
M3 - Article
C2 - 19035641
AN - SCOPUS:58849123376
VL - 56
SP - 12099
EP - 12104
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 24
ER -