TY - JOUR
T1 - Safety evaluation of nanoliposomes containing cyclosporine A after ocular administration
AU - Mosallaei, Navid
AU - Banaee, Touka
AU - Farzadnia, Mehdi
AU - Abedini, Ehsan
AU - Ashraf, Hami
AU - Malaekeh-Nikouei, Bizhan
N1 - Funding Information:
Declaration of interest: This work was supported financially by a research grant from the Vice Chancellor for Research of Mashhad University of Medical Sciences, Mashhad, Iran. Authors declare that there is no conflict of interests in this study.
PY - 2012/6
Y1 - 2012/6
N2 - Aim: Cyclosporine A (CyA) is an anti inflammatory drug which modulates immune system. We prepared two liposomal formulations of CyA for ophthalmic administration. In the present study, the safety of these formulations was tested in rabbits. Materials and methods: Fusogenic and positively charged liposomes were prepared by solvent evaporation method. CyA concentration in ophthalmic preparations was 0.2% w/v. Twenty New Zealand albino rabbits (40 eyes) were divided into two groups. Right eyes were treated and left eyes served as control. Eyes were examined clinically in 1, 3, 7, 14 and 28th days of the study. Eleven eyes were enucleated for pathological evaluation at the end of the study. Results: Mean size and charge of positively charged and fusogenic liposomes were 110.7±1.5 and 114.9±15.2 nm; 19.0±2.2 and 2.5±0.5 mV, respectively. Clinically, there were transient conjunctival injections and corneal epithelial defects in a few cases which were not limited to the treated eyes. Positively charged liposomes caused conjunctival injection in two cases, while this complication occurred in only one eye after administration of fusogenic liposomes. Histopathological examination revealed the presence of follicular conjunctivitis in two eyes with conjunctival injection. Inflammation in cases received the fusogenic form of the drug was mild and non-specific. Conclusions: The results of this study suggest that fusogenic liposomal preparation of CyA is safe and can be useful for future studies.
AB - Aim: Cyclosporine A (CyA) is an anti inflammatory drug which modulates immune system. We prepared two liposomal formulations of CyA for ophthalmic administration. In the present study, the safety of these formulations was tested in rabbits. Materials and methods: Fusogenic and positively charged liposomes were prepared by solvent evaporation method. CyA concentration in ophthalmic preparations was 0.2% w/v. Twenty New Zealand albino rabbits (40 eyes) were divided into two groups. Right eyes were treated and left eyes served as control. Eyes were examined clinically in 1, 3, 7, 14 and 28th days of the study. Eleven eyes were enucleated for pathological evaluation at the end of the study. Results: Mean size and charge of positively charged and fusogenic liposomes were 110.7±1.5 and 114.9±15.2 nm; 19.0±2.2 and 2.5±0.5 mV, respectively. Clinically, there were transient conjunctival injections and corneal epithelial defects in a few cases which were not limited to the treated eyes. Positively charged liposomes caused conjunctival injection in two cases, while this complication occurred in only one eye after administration of fusogenic liposomes. Histopathological examination revealed the presence of follicular conjunctivitis in two eyes with conjunctival injection. Inflammation in cases received the fusogenic form of the drug was mild and non-specific. Conclusions: The results of this study suggest that fusogenic liposomal preparation of CyA is safe and can be useful for future studies.
KW - Cyclosporine A
KW - In vivo study
KW - Liposomes
KW - Ocular administration
KW - Safety
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U2 - 10.3109/02713683.2012.660595
DO - 10.3109/02713683.2012.660595
M3 - Article
C2 - 22577761
AN - SCOPUS:84861037364
SN - 0271-3683
VL - 37
SP - 453
EP - 456
JO - Current Eye Research
JF - Current Eye Research
IS - 6
ER -