Safety evaluation of nanoliposomes containing cyclosporine A after ocular administration

Navid Mosallaei, Touka Banaee, Mehdi Farzadnia, Ehsan Abedini, Hami Ashraf, Bizhan Malaekeh-Nikouei

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Cyclosporine A (CyA) is an anti inflammatory drug which modulates immune system. We prepared two liposomal formulations of CyA for ophthalmic administration. In the present study, the safety of these formulations was tested in rabbits. Materials and methods: Fusogenic and positively charged liposomes were prepared by solvent evaporation method. CyA concentration in ophthalmic preparations was 0.2% w/v. Twenty New Zealand albino rabbits (40 eyes) were divided into two groups. Right eyes were treated and left eyes served as control. Eyes were examined clinically in 1, 3, 7, 14 and 28th days of the study. Eleven eyes were enucleated for pathological evaluation at the end of the study. Results: Mean size and charge of positively charged and fusogenic liposomes were 110.7±1.5 and 114.9±15.2 nm; 19.0±2.2 and 2.5±0.5 mV, respectively. Clinically, there were transient conjunctival injections and corneal epithelial defects in a few cases which were not limited to the treated eyes. Positively charged liposomes caused conjunctival injection in two cases, while this complication occurred in only one eye after administration of fusogenic liposomes. Histopathological examination revealed the presence of follicular conjunctivitis in two eyes with conjunctival injection. Inflammation in cases received the fusogenic form of the drug was mild and non-specific. Conclusions: The results of this study suggest that fusogenic liposomal preparation of CyA is safe and can be useful for future studies.

Original languageEnglish (US)
Pages (from-to)453-456
Number of pages4
JournalCurrent Eye Research
Volume37
Issue number6
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • Cyclosporine A
  • In vivo study
  • Liposomes
  • Ocular administration
  • Safety

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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