Salivary apyrase activity of some old world phlebotomine sand flies

JoséM C. Ribeiro, Govind B. Modi, Robert B. Tesh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Salivary gland homogenates of three Old World phlebotomine sand flies (Phlebotomus papatasi, P. argentipes and P. perniciosus) contained abundant ATPase and ADPase activities, indicating the presence of an apyrase activity. These activities had an optimum pH around 8.0 and were activated by Ca2+ but not Mg2+. Both hydrolytic activities and salivary protein content were significantly reduced after the female sand fly took a blood meal indicating a secretory fate for the enzymic activities and salivary gland contents during the feeding process. In contrast to the above mentioned species, the salivary apyrase activity of P. colabaensis is much less abundant. Salivary gland homogenates of P. papatasi, P. argentipes and P. perniciosus inhibited ADP-induced platelet aggregation of citrated rabbit platelet rich plasma. It is suggested that salivary apyrase activity, as in some other blood-sucking arthropods, helps the blood-feeding process by preventing host platelet aggregation.

Original languageEnglish (US)
Pages (from-to)409-412
Number of pages4
JournalInsect Biochemistry
Volume19
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

apyrase
Apyrase
Psychodidae
Phlebotominae
Sand
Platelets
salivary glands
Salivary Glands
Phlebotomus papatasi
Blood
platelet aggregation
Platelet Aggregation
Phlebotomus argentipes
Agglomeration
Phlebotomus perniciosus
hematophagous arthropods
Phlebotomus
Salivary Proteins and Peptides
Platelet-Rich Plasma
Arthropods

Keywords

  • apyrase
  • feeding
  • haematophagy
  • Phlebotomus argentipes
  • Phlebotomus colabaensis
  • Phlebotomus papatasi
  • Phlebotomus perniciosus
  • platelet aggregation
  • saliva
  • sand fly

Cite this

Salivary apyrase activity of some old world phlebotomine sand flies. / Ribeiro, JoséM C.; Modi, Govind B.; Tesh, Robert B.

In: Insect Biochemistry, Vol. 19, No. 4, 1989, p. 409-412.

Research output: Contribution to journalArticle

Ribeiro, JoséM C. ; Modi, Govind B. ; Tesh, Robert B. / Salivary apyrase activity of some old world phlebotomine sand flies. In: Insect Biochemistry. 1989 ; Vol. 19, No. 4. pp. 409-412.
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