Salmonella typhimurium enterotoxin epitopes shared among bacteria

Ashok K. Chopra, Xin Jing Xu, Johnny W. Peterson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The deduced amino acid sequence of the cloned Salmonella enterotoxin gene (stn) was used to prepare anti-peptide antibodies. These antibodies were then employed to screen isolates of this enteric pathogen for the synthesis of protein enterotoxin (Stn). Cell lysates of all Salmonella isolates tested displayed a prominent immunoblot band of approximately 29 kDa, which was consistent with the size of the cloned stn gene product. Among other Gram-negative bacteria examined, isolates of Klebsiella Enterobacter, and Citrobacter exhibited a similar-sized protein that reacted strongly with the Stn antibodies. Since the stn gene was located opposite the hydrogenase regulatory genes (hydHG) required for hydrogen metabolism in bacteria, our data suggested that only in Salmonella and some other members of the family Enterobacteriaceae had the DNA sequence evolved, presumably through point mutations, into an expressed gene product of similar size.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalFEMS Microbiology Letters
Volume118
Issue number3
DOIs
StatePublished - May 15 1994

Keywords

  • Anti-peptide antibodies
  • Immunoblot analysis
  • Salmonella typhimurium
  • stn gene

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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