Salmonella typhimurium enterotoxin epitopes shared among bacteria

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The deduced amino acid sequence of the cloned Salmonella enterotoxin gene (stn) was used to prepare anti-peptide antibodies. These antibodies were then employed to screen isolates of this enteric pathogen for the synthesis of protein enterotoxin (Stn). Cell lysates of all Salmonella isolates tested displayed a prominent immunoblot band of approximately 29 kDa, which was consistent with the size of the cloned stn gene product. Among other Gram-negative bacteria examined, isolates of Klebsiella Enterobacter, and Citrobacter exhibited a similar-sized protein that reacted strongly with the Stn antibodies. Since the stn gene was located opposite the hydrogenase regulatory genes (hydHG) required for hydrogen metabolism in bacteria, our data suggested that only in Salmonella and some other members of the family Enterobacteriaceae had the DNA sequence evolved, presumably through point mutations, into an expressed gene product of similar size.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalFEMS Microbiology Letters
Volume118
Issue number3
StatePublished - May 15 1994

Fingerprint

Salmonella typhimurium
Epitopes
Bacteria
Salmonella
Genes
Citrobacter
Hydrogenase
Enterobacter
Klebsiella
Antibodies
Enterotoxins
Enterobacteriaceae
Regulator Genes
Gram-Negative Bacteria
Point Mutation
Hydrogen
Anti-Idiotypic Antibodies
Amino Acid Sequence
Proteins
Peptides

Keywords

  • Anti-peptide antibodies
  • Immunoblot analysis
  • Salmonella typhimurium
  • stn gene

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Salmonella typhimurium enterotoxin epitopes shared among bacteria. / Chopra, Ashok; Xu, Xin Jing; Peterson, Johnny.

In: FEMS Microbiology Letters, Vol. 118, No. 3, 15.05.1994, p. 237-242.

Research output: Contribution to journalArticle

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