SARS-Like coronavirus WIV1-CoV does not replicate in Egyptian fruit bats (Rousettus aegyptiacus)

Neeltje van Doremalen; Alexandra Schäfer; Vineet D. Menachery; Michael Letko; Trenton Bushmaker; Robert J. Fischer; Dania M. Figueroa; Patrick W. Hanley; Greg Saturday; Ralph S. Baric; Vincent J. Munster

doi:10.3390/v10120727

SARS-Like coronavirus WIV1-CoV does not replicate in Egyptian fruit bats (Rousettus aegyptiacus)

Neeltje van Doremalen, Alexandra Schäfer, Vineet D. Menachery, Michael Letko, Trenton Bushmaker, Robert J. Fischer, Dania M. Figueroa, Patrick W. Hanley, Greg Saturday, Ralph S. Baric, Vincent J. Munster

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Severe acute respiratory syndrome (SARS)-like WIV1-coronavirus (CoV) was first isolated from Rhinolophus sinicus bats and can use the human angiotensin converting enzyme 2 (ACE2) receptor. In the current study, we investigate the ability of WIV1-CoV to infect Rousettus aegyptiacus bats. No clinical signs were observed throughout the experiment. Furthermore, only four oropharyngeal swabs and two respiratory tissues, isolated on day 3 post inoculation, were found positive for viral RNA. Two out of twelve bats showed a modest increase in coronavirus specific antibodies post challenge. In conclusion, WIV1-CoV was unable to cause a robust infection in Rousettus aegyptiacus bats.

Original language	English (US)
Article number	727
Journal	Viruses
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/v10120727
State	Published - Dec 2018
Externally published	Yes

Keywords

Animal model
Coronavirus
Emerging infectious diseases
WIV1-CoV

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v10120727

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title = "SARS-Like coronavirus WIV1-CoV does not replicate in Egyptian fruit bats (Rousettus aegyptiacus)",

abstract = "Severe acute respiratory syndrome (SARS)-like WIV1-coronavirus (CoV) was first isolated from Rhinolophus sinicus bats and can use the human angiotensin converting enzyme 2 (ACE2) receptor. In the current study, we investigate the ability of WIV1-CoV to infect Rousettus aegyptiacus bats. No clinical signs were observed throughout the experiment. Furthermore, only four oropharyngeal swabs and two respiratory tissues, isolated on day 3 post inoculation, were found positive for viral RNA. Two out of twelve bats showed a modest increase in coronavirus specific antibodies post challenge. In conclusion, WIV1-CoV was unable to cause a robust infection in Rousettus aegyptiacus bats.",

keywords = "Animal model, Coronavirus, Emerging infectious diseases, WIV1-CoV",

author = "{van Doremalen}, Neeltje and Alexandra Sch{\"a}fer and Menachery, {Vineet D.} and Michael Letko and Trenton Bushmaker and Fischer, {Robert J.} and Figueroa, {Dania M.} and Hanley, {Patrick W.} and Greg Saturday and Baric, {Ralph S.} and Munster, {Vincent J.}",

note = "Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2018",

month = dec,

doi = "10.3390/v10120727",

language = "English (US)",

volume = "10",

journal = "Viruses",

issn = "1999-4915",

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AU - van Doremalen, Neeltje

AU - Schäfer, Alexandra

AU - Menachery, Vineet D.

AU - Letko, Michael

AU - Bushmaker, Trenton

AU - Fischer, Robert J.

AU - Figueroa, Dania M.

AU - Hanley, Patrick W.

AU - Saturday, Greg

AU - Baric, Ralph S.

AU - Munster, Vincent J.

PY - 2018/12

Y1 - 2018/12

N2 - Severe acute respiratory syndrome (SARS)-like WIV1-coronavirus (CoV) was first isolated from Rhinolophus sinicus bats and can use the human angiotensin converting enzyme 2 (ACE2) receptor. In the current study, we investigate the ability of WIV1-CoV to infect Rousettus aegyptiacus bats. No clinical signs were observed throughout the experiment. Furthermore, only four oropharyngeal swabs and two respiratory tissues, isolated on day 3 post inoculation, were found positive for viral RNA. Two out of twelve bats showed a modest increase in coronavirus specific antibodies post challenge. In conclusion, WIV1-CoV was unable to cause a robust infection in Rousettus aegyptiacus bats.

AB - Severe acute respiratory syndrome (SARS)-like WIV1-coronavirus (CoV) was first isolated from Rhinolophus sinicus bats and can use the human angiotensin converting enzyme 2 (ACE2) receptor. In the current study, we investigate the ability of WIV1-CoV to infect Rousettus aegyptiacus bats. No clinical signs were observed throughout the experiment. Furthermore, only four oropharyngeal swabs and two respiratory tissues, isolated on day 3 post inoculation, were found positive for viral RNA. Two out of twelve bats showed a modest increase in coronavirus specific antibodies post challenge. In conclusion, WIV1-CoV was unable to cause a robust infection in Rousettus aegyptiacus bats.

KW - Animal model

KW - Coronavirus

KW - Emerging infectious diseases

KW - WIV1-CoV

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SARS-Like coronavirus WIV1-CoV does not replicate in Egyptian fruit bats (Rousettus aegyptiacus)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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