Scanning electron microscopy of the subarachnoid space in the dog. I. Spinal cord levels

M. W. Cloyd, F. N. Low

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Young dogs 6 to 14 weeks old were perfused with aldehydes by standard techniques followed by Microfil to prevent blood vessel collapse. Tissue pieces possessing natural surfaces of the subarachnoid space at spinal cord levels were removed, post osmicated, dehydrated, critical point dried, coated with palladium gold and examined in a Cambridge Stereoscan S4 scanning electron microscope. The surfaces of the spinal pia mater and arachnoid are clearly expressions of connective tissues with fenestrations and underlying connective tissue fibrils. Fenestrations are especially common in the conus medullaris where connective tissue fibrils are more likely exposed to cerebrospinal fluid than covered by pial lining cells. Long and short microvillae and blebs are present in profusion and with distributions comparable to the same structures on other cells. The surfaces of traversing structures - blood vessels, nerve roots, arachnoid trabeculae and denticulate ligaments - are covered with tissues comparable to and continuous with pia and arachnoid despite underlying differences in histological makeup. Free cells are present on all spinal leptomeningeal surfaces in surprising profusion. They exhibit extreme pleomorphism of surface contours which is comparable to that observed in the scanning electron microscopy of cells from other sources. It is highly probable that these free cells are macrophages.

Original languageEnglish (US)
Pages (from-to)325-368
Number of pages44
JournalJournal of Comparative Neurology
Volume153
Issue number4
StatePublished - 1974

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Subarachnoid Space
Electron Scanning Microscopy
Spinal Cord
Arachnoid
Dogs
Connective Tissue
Blood Vessels
Pia Mater
Silicone Elastomers
Palladium
Blister
Ligaments
Aldehydes
Gold
Cerebrospinal Fluid
Macrophages
Electrons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Scanning electron microscopy of the subarachnoid space in the dog. I. Spinal cord levels. / Cloyd, M. W.; Low, F. N.

In: Journal of Comparative Neurology, Vol. 153, No. 4, 1974, p. 325-368.

Research output: Contribution to journalArticle

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