Schwann cell nerve growth factor receptor expression during initiation of remyelination

X. Fan, Benjamin Gelman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Initiation of remyelination is a promising therapeutic strategy to treat patients with demyelinating diseases, but specific factors that control remyelination are not clear. We first reported that expression of nerve growth factor receptor (NGFR) was increased during initiation of remyelination (Fan and Gelman, Journal of Neuropathology and Experimental Neurology 49:312, 1990). In this study, we characterized the timing and cellular localization of NGFR expression in a model of segmental demyelination and remyelination using immunohistochemistry and monoclonal antibody 192-IgG, and compared it to an axonal neuropathy. At the onset of demyelination induced by tellurium (Te) poisoning, NGFR antigenicity was selectively expressed within and around demyelinating internodes in rat sciatic nerve. Dual fluorescence staining with myelin-specific antigen showed that NGFR colocalized with demyelinated internodal units with relative specificity; Schwann cell S-100 protein showed a concomitant down-regulation in injured internodes. Peak expression of NGFR occurred during the transition between demyelination and remyelination (day 8 of Te), then declined exponentially. NGFR expression was most prominent in the cytoplasm of daughter Schwann cells as they established contact with denuded axons, and was sharply repressed as compact myelin began to accumulate. Rare colocalization with neurofilament antigens revealed intraaxonal deposits of NGFR in segmental demyelination. In the nerve crush model, Schwann cell NGFR expression was not segmentally distributed and was upregulated for a longer period of time. Our data establish that NGFR expression in the peripheral nervous system is not strictly linked to axon elongation, and that it probably functions during the initiation of myelination.

Original languageEnglish (US)
Pages (from-to)58-67
Number of pages10
JournalJournal of Neuroscience Research
Volume31
Issue number1
StatePublished - 1992

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Nerve Growth Factor Receptor
Schwann Cells
Demyelinating Diseases
Tellurium
Myelin Sheath
Axons
Nerve Crush
Antigens
Intermediate Filaments
S100 Proteins
Peripheral Nervous System
Sciatic Nerve
Neurology
Poisoning
Cytoplasm
Down-Regulation
Immunoglobulin G
Fluorescence
Immunohistochemistry
Monoclonal Antibodies

Keywords

  • NGF receptor
  • S-100 protein
  • segmental demyelination
  • tellurium neuropathy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Schwann cell nerve growth factor receptor expression during initiation of remyelination. / Fan, X.; Gelman, Benjamin.

In: Journal of Neuroscience Research, Vol. 31, No. 1, 1992, p. 58-67.

Research output: Contribution to journalArticle

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