Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination

K. Reddisiva Prasanth, Nikolay Kovalev, Isabel Fernández de Castro Martín, Jannine Baker, Peter D. Nagy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Genetic recombination in RNA viruses drives the evolutionary arms race with host's antiviral strategies and recombination also facilitates adaptation of viruses to new hosts. In this paper, the authors used tombusvirus and a temperature-sensitive (ts) mutant library of yeast to identify 40 host proteins affecting viral recombination in yeast model host. Subsequent detailed analysis with two identified actin-related proteins, Act1p and Arp3p, has revealed that the wt actin network helps TBSV to maintain low level viral recombination. Pharmacological inhibition of actin in plant protoplasts confirmed the role of the actin network in tombusvirus recombination. An in vitro approach revealed the altered activity of the tombusvirus replicase in the presence of mutated Act1p. The authors show more efficient recruitment of a cellular DEAD-box helicase, which enhances tombusvirus recombination, into the membrane-bound replicase in Act1p mutant yeast. Overall, this work shows that the actin network affects tombusvirus recombination in yeast and plant cells.

Original languageEnglish (US)
Pages (from-to)233-242
Number of pages10
JournalVirology
Volume489
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

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Tombusvirus
Genetic Recombination
Libraries
Actins
Yeasts
RNA
Temperature
Protoplasts
RNA Viruses
Plant Cells
Viral Proteins
Antiviral Agents
Pharmacology
Viruses
Membranes

ASJC Scopus subject areas

  • Virology

Cite this

Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination. / Prasanth, K. Reddisiva; Kovalev, Nikolay; de Castro Martín, Isabel Fernández; Baker, Jannine; Nagy, Peter D.

In: Virology, Vol. 489, 01.02.2016, p. 233-242.

Research output: Contribution to journalArticle

Prasanth, K. Reddisiva ; Kovalev, Nikolay ; de Castro Martín, Isabel Fernández ; Baker, Jannine ; Nagy, Peter D. / Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination. In: Virology. 2016 ; Vol. 489. pp. 233-242.
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