Screening of lysyl oxidase (LOX) and lysyl oxidase like (LOXL) enzyme expression and activity in preterm prelabor rupture of fetal membranes

Jossimara Polettini, Marcia G. Silva, Marian Kacerovsky, Tariq A. Syed, George Saade, Ramkumar Menon

    Research output: Contribution to journalArticle

    10 Scopus citations

    Abstract

    Objective: Lysyl oxidase (LOX) and LOX like enzymes (LOXL1-4) physiologically remodel extracellular matrix and pathologically contribute to cellular senescence under oxidative stress (OS). We characterized LOX and LOXL expressions and activity in human fetal membranes. Methods: Human fetal membranes from women with uncomplicated pregnancies at term, preterm birth with intact membranes (PTB) or preterm prelabor rupture of membranes (pPROM), and in vitro fetal membranes stimulated with water-soluble cigarette smoke extract (CSE), an OS inducer, were analyzed by real-time PCR and immunohistochemistry for LOX and LOXL (1-4) expression and localization. LOX activity was measured by fluorometric assay. Results: LOX gene expression was ∼2.5-fold higher in fetal membranes from pPROM compared to PTB and term (P=0.02). LOX and LOXL1, 2 and 4 were localized to both amniotic and chorionic cells, whereas LOXL3 was limited to chorion. LOX and LOXL isoform expressions were not different between CSE treated and untreated groups, while LOX activity was increased in the presence of an antioxidant (P=0.02). Conclusions: Increase of LOX expression in pPROM, an OS-related disease, and the apparent inhibition of LOX activity by CSE restored by antioxidant treatment suggest that reactive oxygen species might influence LOX-mediated tissue remodeling in fetal membranes. Balanced antioxidant supplementation during pregnancy may reduce the risk of pPROM by increasing LOX activity.

    Original languageEnglish (US)
    Pages (from-to)99-109
    Number of pages11
    JournalJournal of Perinatal Medicine
    Volume44
    Issue number1
    DOIs
    StatePublished - Jan 1 2016

    Keywords

    • Antioxidant
    • cigarette smoke
    • oxidative stress
    • preterm birth

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Pediatrics, Perinatology, and Child Health

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