Search for MicroRNAs expressed by intracellular bacterial pathogens in infected mammalian cells

Yuki Furuse, Ryan Finethy, Hector A. Saka, Ana M. Xet-Mull, Dana M. Sisk, Kristen L. Jurcic Smith, Sunhee Lee, Jörn Coers, Raphael H. Valdivia, David M. Tobin, Bryan R. Cullen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

MicroRNAs are expressed by all multicellular organisms and play a critical role as post-transcriptional regulators of gene expression. Moreover, different microRNA species are known to influence the progression of a range of different diseases, including cancer and microbial infections. A number of different human viruses also encode microRNAs that can attenuate cellular innate immune responses and promote viral replication, and a fungal pathogen that infects plants has recently been shown to express microRNAs in infected cells that repress host cell immune responses and promote fungal pathogenesis. Here, we have used deep sequencing of total expressed small RNAs, as well as small RNAs associated with the cellular RNA-induced silencing complex RISC, to search for microRNAs that are potentially expressed by intracellular bacterial pathogens and translocated into infected animal cells. In the case of Legionella and Chlamydia and the two mycobacterial species M. smegmatis and M. tuberculosis, we failed to detect any bacterial small RNAs that had the characteristics expected for authentic microRNAs, although large numbers of small RNAs of bacterial origin could be recovered. However, a third mycobacterial species, M. marinum, did express an ∼23-nt small RNA that was bound by RISC and derived from an RNA stem-loop with the characteristics expected for a pre-microRNA. While intracellular expression of this candidate bacterial microRNA was too low to effectively repress target mRNA species in infected cultured cells in vitro, artificial overexpression of this potential bacterial pre-microRNA did result in the efficient repression of a target mRNA. This bacterial small RNA therefore represents the first candidate microRNA of bacterial origin.

Original languageEnglish (US)
Article numbere106434
JournalPloS one
Volume9
Issue number9
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Pathogens
MicroRNAs
microRNA
Cells
pathogens
RNA
Bacterial RNA
cells
Reduced instruction set computing
RNA-Induced Silencing Complex
Smegma
Legionella
vertebrate viruses
High-Throughput Nucleotide Sequencing
Messenger RNA
Chlamydia
Regulator Genes
virus replication
regulator genes
Viruses

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Furuse, Y., Finethy, R., Saka, H. A., Xet-Mull, A. M., Sisk, D. M., Jurcic Smith, K. L., ... Cullen, B. R. (2014). Search for MicroRNAs expressed by intracellular bacterial pathogens in infected mammalian cells. PloS one, 9(9), [e106434]. https://doi.org/10.1371/journal.pone.0106434

Search for MicroRNAs expressed by intracellular bacterial pathogens in infected mammalian cells. / Furuse, Yuki; Finethy, Ryan; Saka, Hector A.; Xet-Mull, Ana M.; Sisk, Dana M.; Jurcic Smith, Kristen L.; Lee, Sunhee; Coers, Jörn; Valdivia, Raphael H.; Tobin, David M.; Cullen, Bryan R.

In: PloS one, Vol. 9, No. 9, e106434, 01.01.2014.

Research output: Contribution to journalArticle

Furuse, Y, Finethy, R, Saka, HA, Xet-Mull, AM, Sisk, DM, Jurcic Smith, KL, Lee, S, Coers, J, Valdivia, RH, Tobin, DM & Cullen, BR 2014, 'Search for MicroRNAs expressed by intracellular bacterial pathogens in infected mammalian cells', PloS one, vol. 9, no. 9, e106434. https://doi.org/10.1371/journal.pone.0106434
Furuse Y, Finethy R, Saka HA, Xet-Mull AM, Sisk DM, Jurcic Smith KL et al. Search for MicroRNAs expressed by intracellular bacterial pathogens in infected mammalian cells. PloS one. 2014 Jan 1;9(9). e106434. https://doi.org/10.1371/journal.pone.0106434
Furuse, Yuki ; Finethy, Ryan ; Saka, Hector A. ; Xet-Mull, Ana M. ; Sisk, Dana M. ; Jurcic Smith, Kristen L. ; Lee, Sunhee ; Coers, Jörn ; Valdivia, Raphael H. ; Tobin, David M. ; Cullen, Bryan R. / Search for MicroRNAs expressed by intracellular bacterial pathogens in infected mammalian cells. In: PloS one. 2014 ; Vol. 9, No. 9.
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