Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Yan Li, Ying Lei Wong, Michelle Yueqi Lee, Qingxin Li, Qing Yin Wang, Julien Lescar, Pei-Yong Shi, Cong Bao Kang

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.

Original languageEnglish (US)
Pages (from-to)12068-12072
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number39
DOIs
StatePublished - Sep 19 2016

Keywords

  • dengue virus
  • membrane protein
  • membrane topology
  • NMR spectroscopy
  • NS4B

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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