Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Yan Li, Ying Lei Wong, Michelle Yueqi Lee, Qingxin Li, Qing Yin Wang, Julien Lescar, Pei-Yong Shi, Cong Bao Kang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.

Original languageEnglish (US)
Pages (from-to)12068-12072
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number39
DOIs
StatePublished - Sep 19 2016

Fingerprint

Micelles
Viruses
Topology
Proteins
Membranes
Phosphatidylglycerols
Membrane Proteins
Nuclear magnetic resonance
Pharmaceutical Preparations
Experiments

Keywords

  • dengue virus
  • membrane protein
  • membrane topology
  • NMR spectroscopy
  • NS4B

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Li, Y., Wong, Y. L., Lee, M. Y., Li, Q., Wang, Q. Y., Lescar, J., ... Kang, C. B. (2016). Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles. Angewandte Chemie - International Edition, 55(39), 12068-12072. https://doi.org/10.1002/anie.201606609

Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles. / Li, Yan; Wong, Ying Lei; Lee, Michelle Yueqi; Li, Qingxin; Wang, Qing Yin; Lescar, Julien; Shi, Pei-Yong; Kang, Cong Bao.

In: Angewandte Chemie - International Edition, Vol. 55, No. 39, 19.09.2016, p. 12068-12072.

Research output: Contribution to journalArticle

Li, Yan ; Wong, Ying Lei ; Lee, Michelle Yueqi ; Li, Qingxin ; Wang, Qing Yin ; Lescar, Julien ; Shi, Pei-Yong ; Kang, Cong Bao. / Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles. In: Angewandte Chemie - International Edition. 2016 ; Vol. 55, No. 39. pp. 12068-12072.
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