Secondary structure prediction of the H5 pore of potassium channels

K. V. Soman, J. A. McCammon, A. M. Brown

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The 'H5' segment located between the putative fifth and sixth transmembrane helices is the most highly conserved region in voltage-gated potassium channels and it is believed to constitute a major part of the ion conduction path (pore). Here we present a two-step procedure, comprising secondary structure prediction and hydrophobic moment profiling, to predict the structure of this important region. Combined results from the application of the procedure to the H5 region of four voltage-gated and five other K+ channel sequences lead to the prediction of a β strand-turn-β-strand structure for H5. The reasons for the application of these soluble protein methods to parts of membrane proteins are (i) that pore-lining residues are accessible to water and (ii) that a large enough database of high resolution membrane protein structures does not yet exist. The results are compared with experimental results, in particular spectroscopic studies of two peptides based on the H5 sequence of SHAKER potassium channel. The procedure developed here may be applicable to water-accessible regions of other membrane proteins.

Original languageEnglish (US)
Pages (from-to)397-401
Number of pages5
JournalProtein Engineering
Volume8
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Potassium Channels
Potassium
Membrane Proteins
Proteins
Membranes
Voltage-Gated Potassium Channels
Water
Linings
Electric potential
Ions
Peptides
Databases

Keywords

  • Hydrophobic moment
  • Membrane protein
  • Pore model
  • Potassium channel
  • Protein structure prediction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Soman, K. V., McCammon, J. A., & Brown, A. M. (1995). Secondary structure prediction of the H5 pore of potassium channels. Protein Engineering, 8(4), 397-401.

Secondary structure prediction of the H5 pore of potassium channels. / Soman, K. V.; McCammon, J. A.; Brown, A. M.

In: Protein Engineering, Vol. 8, No. 4, 1995, p. 397-401.

Research output: Contribution to journalArticle

Soman, KV, McCammon, JA & Brown, AM 1995, 'Secondary structure prediction of the H5 pore of potassium channels', Protein Engineering, vol. 8, no. 4, pp. 397-401.
Soman, K. V. ; McCammon, J. A. ; Brown, A. M. / Secondary structure prediction of the H5 pore of potassium channels. In: Protein Engineering. 1995 ; Vol. 8, No. 4. pp. 397-401.
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