Secretion of Interferon by Bacillus subtilis

Ilkka Palva; Päivi Lehtovaara; Leevi Kääriäinen; Mervi Sibakov; Kari Cantell; Catherine H. Schein; Kiyoko Kashiwagi; Charles Weissmann

doi:10.1016/0378-1119(83)90107-5

Secretion of Interferon by Bacillus subtilis

Ilkka Palva, Päivi Lehtovaara, Leevi Kääriäinen, Mervi Sibakov, Kari Cantell, Catherine H. Schein, Kiyoko Kashiwagi, Charles Weissmann

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Bacillus subtilis was transformed with a hybrid gene in which the sequence encoding the α-amylase signal peptide was joined by a linker to the sequence encoding mature human interferon α2 (IFN-α2). The hybrid preprotein was cleaved precisely following the last amino acid of the α-amylase signal sequence and was secreted at 0.5-1 mg per liter. IFN-α2, preceded by either one or six amino acids, has the same specific antiviral activity as IFN-α2 itself.

Original language	English (US)
Pages (from-to)	229-235
Number of pages	7
Journal	Gene
Volume	22
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(83)90107-5
State	Published - 1983
Externally published	Yes

Keywords

antiviral activity
preprotein
Recombinant DNA
α-amylase signal peptide

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(83)90107-5

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title = "Secretion of Interferon by Bacillus subtilis",

abstract = "Bacillus subtilis was transformed with a hybrid gene in which the sequence encoding the α-amylase signal peptide was joined by a linker to the sequence encoding mature human interferon α2 (IFN-α2). The hybrid preprotein was cleaved precisely following the last amino acid of the α-amylase signal sequence and was secreted at 0.5-1 mg per liter. IFN-α2, preceded by either one or six amino acids, has the same specific antiviral activity as IFN-α2 itself.",

keywords = "antiviral activity, preprotein, Recombinant DNA, α-amylase signal peptide",

author = "Ilkka Palva and P{\"a}ivi Lehtovaara and Leevi K{\"a}{\"a}ri{\"a}inen and Mervi Sibakov and Kari Cantell and Schein, {Catherine H.} and Kiyoko Kashiwagi and Charles Weissmann",

year = "1983",

doi = "10.1016/0378-1119(83)90107-5",

language = "English (US)",

volume = "22",

pages = "229--235",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "2-3",

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TY - JOUR

T1 - Secretion of Interferon by Bacillus subtilis

AU - Palva, Ilkka

AU - Lehtovaara, Päivi

AU - Kääriäinen, Leevi

AU - Sibakov, Mervi

AU - Cantell, Kari

AU - Schein, Catherine H.

AU - Kashiwagi, Kiyoko

AU - Weissmann, Charles

PY - 1983

Y1 - 1983

N2 - Bacillus subtilis was transformed with a hybrid gene in which the sequence encoding the α-amylase signal peptide was joined by a linker to the sequence encoding mature human interferon α2 (IFN-α2). The hybrid preprotein was cleaved precisely following the last amino acid of the α-amylase signal sequence and was secreted at 0.5-1 mg per liter. IFN-α2, preceded by either one or six amino acids, has the same specific antiviral activity as IFN-α2 itself.

AB - Bacillus subtilis was transformed with a hybrid gene in which the sequence encoding the α-amylase signal peptide was joined by a linker to the sequence encoding mature human interferon α2 (IFN-α2). The hybrid preprotein was cleaved precisely following the last amino acid of the α-amylase signal sequence and was secreted at 0.5-1 mg per liter. IFN-α2, preceded by either one or six amino acids, has the same specific antiviral activity as IFN-α2 itself.

KW - antiviral activity

KW - preprotein

KW - Recombinant DNA

KW - α-amylase signal peptide

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DO - 10.1016/0378-1119(83)90107-5

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AN - SCOPUS:0020505146

SN - 0378-1119

VL - 22

SP - 229

EP - 235

JO - Gene

JF - Gene

IS - 2-3

ER -

Secretion of Interferon by Bacillus subtilis

Abstract

Keywords

ASJC Scopus subject areas

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