SELDI protein expression profiling & biomarker identification of Streptococcus pneumoniae

Utpal Pandya, David Niesel, TuAnh Dang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A protein chip-based proteomics profiling platform employing SELDI-TOF mass spectrometry was used to identify differentially expressed proteins of Streptococcus pneumoniae. The bacterial pellets from the 100 ml culture were washed and resuspended in 5ml PBS supplemented with 1mM PMSF prior to sonication. The clarified cell lysates were normalized and then fractionated on protein chips for differential expression profiling. The protein chip arrays had different chramotographic properties such as metal affinity (IMAC), cation exchange (WCX2), and hydrophobic phase (H4). Profound analysis identified the 57 kDa protein as a regulatory protein based on 10 peptide matches, representing 38% of the sequence, to a putative histidine kinase from S. pyogenes.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages243-244
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Biomarkers
Proteins
Sonication
Histidine
Mass spectrometry
Cations
Phosphotransferases
Metals
Peptides

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Pandya, U., Niesel, D., & Dang, T. (2002). SELDI protein expression profiling & biomarker identification of Streptococcus pneumoniae. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 243-244)

SELDI protein expression profiling & biomarker identification of Streptococcus pneumoniae. / Pandya, Utpal; Niesel, David; Dang, TuAnh.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 243-244.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pandya, U, Niesel, D & Dang, T 2002, SELDI protein expression profiling & biomarker identification of Streptococcus pneumoniae. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 243-244, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Pandya U, Niesel D, Dang T. SELDI protein expression profiling & biomarker identification of Streptococcus pneumoniae. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 243-244
Pandya, Utpal ; Niesel, David ; Dang, TuAnh. / SELDI protein expression profiling & biomarker identification of Streptococcus pneumoniae. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 243-244
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