Selective activation of protein kinase C delta in human neutrophils following ischemia reperfusion of skeletal muscle.

Ruksana Huda, Leoncio A. Vergara, Daneshvari R. Solanki, Edward R. Sherwood, Mali Mathru

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Circulatory neutrophils are known to be critical mediators of inflammation and oxidative stress during ischemia reperfusion (I/R) injury. Recent studies have shown an important role for protein kinase C (PKC) in neutrophil survival and function. Activation of specific isotypes of PKC are known to be involved in membrane alteration and motility, oxidative phosphorylation, and apoptosis modulation of neutrophils. However, the role of PKC in neutrophil responses to I/R in the clinical setting has not been studied. In this study, we examined the neutrophil activation of PKC induced by tourniquet-controlled I/R of skeletal muscle in humans. We found that I/R rapidly activates and translocates PKC delta, but not any of the classical forms of PKC (alpha or beta) from cytosol to the particulate fraction of neutrophils. Particulate translocation of PKC delta is sustained up to 4 h after reperfusion and is associated with kinase activity. Postreperfusion activation of PKC delta in neutrophils signals proapoptosis, but does not cause immediate cell death (as revealed by neutrophil morphology study and DNA-laddering assay). This study indicates that calcium-independent novel PKC delta (nPKC delta) might be predominantly involved in regulating membrane functions and survival of neutrophils associated with post-I/R-induced inflammatory oxidative stress.

Original languageEnglish (US)
Pages (from-to)500-504
Number of pages5
JournalShock (Augusta, Ga.)
Volume21
Issue number6
StatePublished - Jun 2004

Fingerprint

Reperfusion
Skeletal Muscle
Neutrophils
Ischemia
Protein Kinase C-delta
Protein Kinase C
Oxidative Stress
Protein Kinase C beta
Protein Kinase C-alpha
Inflammation Mediators
Tourniquets
Neutrophil Activation
Membranes
human PRKCD protein
Oxidative Phosphorylation
Reperfusion Injury
Cytosol
Cell Death
Phosphotransferases
Apoptosis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Huda, R., Vergara, L. A., Solanki, D. R., Sherwood, E. R., & Mathru, M. (2004). Selective activation of protein kinase C delta in human neutrophils following ischemia reperfusion of skeletal muscle. Shock (Augusta, Ga.), 21(6), 500-504.

Selective activation of protein kinase C delta in human neutrophils following ischemia reperfusion of skeletal muscle. / Huda, Ruksana; Vergara, Leoncio A.; Solanki, Daneshvari R.; Sherwood, Edward R.; Mathru, Mali.

In: Shock (Augusta, Ga.), Vol. 21, No. 6, 06.2004, p. 500-504.

Research output: Contribution to journalArticle

Huda, R, Vergara, LA, Solanki, DR, Sherwood, ER & Mathru, M 2004, 'Selective activation of protein kinase C delta in human neutrophils following ischemia reperfusion of skeletal muscle.', Shock (Augusta, Ga.), vol. 21, no. 6, pp. 500-504.
Huda, Ruksana ; Vergara, Leoncio A. ; Solanki, Daneshvari R. ; Sherwood, Edward R. ; Mathru, Mali. / Selective activation of protein kinase C delta in human neutrophils following ischemia reperfusion of skeletal muscle. In: Shock (Augusta, Ga.). 2004 ; Vol. 21, No. 6. pp. 500-504.
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