Selective biomolecular nanoarrays for parallel single-molecule investigations

Matteo Palma; Justin J. Abramson; Alon A. Gorodetsky; Erika Penzo; Ruben L. Gonzalez; Michael P. Sheetz; Colin Nuckolls; James Hone; Shalom J. Wind

doi:10.1021/ja201031g

Selective biomolecular nanoarrays for parallel single-molecule investigations

Matteo Palma, Justin J. Abramson, Alon A. Gorodetsky, Erika Penzo, Ruben L. Gonzalez, Michael P. Sheetz, Colin Nuckolls, James Hone, Shalom J. Wind

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.

Original language	English (US)
Pages (from-to)	7656-7659
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	20
DOIs	https://doi.org/10.1021/ja201031g
State	Published - May 25 2011
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja201031g

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abstract = "The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.",

author = "Matteo Palma and Abramson, {Justin J.} and Gorodetsky, {Alon A.} and Erika Penzo and Gonzalez, {Ruben L.} and Sheetz, {Michael P.} and Colin Nuckolls and James Hone and Wind, {Shalom J.}",

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AU - Palma, Matteo

AU - Abramson, Justin J.

AU - Gorodetsky, Alon A.

AU - Penzo, Erika

AU - Gonzalez, Ruben L.

AU - Sheetz, Michael P.

AU - Nuckolls, Colin

AU - Hone, James

AU - Wind, Shalom J.

PY - 2011/5/25

Y1 - 2011/5/25

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AB - The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.

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Selective biomolecular nanoarrays for parallel single-molecule investigations

Abstract

ASJC Scopus subject areas

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