Selective biomolecular nanoarrays for parallel single-molecule investigations

Matteo Palma, Justin J. Abramson, Alon A. Gorodetsky, Erika Penzo, Ruben L. Gonzalez, Michael Sheetz, Colin Nuckolls, James Hone, Shalom J. Wind

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.

Original languageEnglish (US)
Pages (from-to)7656-7659
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number20
DOIs
StatePublished - May 25 2011
Externally publishedYes

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Environmental Monitoring
DNA-Binding Proteins
Self assembly
Adsorption
Molecules
Biomolecules
Bioactivity
Screening
DNA
Throughput
Proteins
Fabrication
Monitoring
Substrates

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Palma, M., Abramson, J. J., Gorodetsky, A. A., Penzo, E., Gonzalez, R. L., Sheetz, M., ... Wind, S. J. (2011). Selective biomolecular nanoarrays for parallel single-molecule investigations. Journal of the American Chemical Society, 133(20), 7656-7659. https://doi.org/10.1021/ja201031g

Selective biomolecular nanoarrays for parallel single-molecule investigations. / Palma, Matteo; Abramson, Justin J.; Gorodetsky, Alon A.; Penzo, Erika; Gonzalez, Ruben L.; Sheetz, Michael; Nuckolls, Colin; Hone, James; Wind, Shalom J.

In: Journal of the American Chemical Society, Vol. 133, No. 20, 25.05.2011, p. 7656-7659.

Research output: Contribution to journalArticle

Palma, M, Abramson, JJ, Gorodetsky, AA, Penzo, E, Gonzalez, RL, Sheetz, M, Nuckolls, C, Hone, J & Wind, SJ 2011, 'Selective biomolecular nanoarrays for parallel single-molecule investigations', Journal of the American Chemical Society, vol. 133, no. 20, pp. 7656-7659. https://doi.org/10.1021/ja201031g
Palma, Matteo ; Abramson, Justin J. ; Gorodetsky, Alon A. ; Penzo, Erika ; Gonzalez, Ruben L. ; Sheetz, Michael ; Nuckolls, Colin ; Hone, James ; Wind, Shalom J. / Selective biomolecular nanoarrays for parallel single-molecule investigations. In: Journal of the American Chemical Society. 2011 ; Vol. 133, No. 20. pp. 7656-7659.
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