Selective biomolecular nanoarrays for parallel single-molecule investigations

Matteo Palma, Justin J. Abramson, Alon A. Gorodetsky, Erika Penzo, Ruben L. Gonzalez, Michael Sheetz, Colin Nuckolls, James Hone, Shalom J. Wind

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.

Original languageEnglish (US)
Pages (from-to)7656-7659
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number20
DOIs
StatePublished - May 25 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Palma, M., Abramson, J. J., Gorodetsky, A. A., Penzo, E., Gonzalez, R. L., Sheetz, M., Nuckolls, C., Hone, J., & Wind, S. J. (2011). Selective biomolecular nanoarrays for parallel single-molecule investigations. Journal of the American Chemical Society, 133(20), 7656-7659. https://doi.org/10.1021/ja201031g