Selective cleavage of an antibiotic drug molecule from peptides and proteins in the gas phase by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry

Clifton K. Fagerquist, Robert R. Hudgins, Mark Emmett, Kristina Håkansson, Alan G. Marshall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The selective cleavage of an antibiotic drug molecule was investigated from peptides and proteins in the gas phase by electron capture dissociation (ECD) FTICR mass spectrometry. DFC from plasma extracts was reacted with the cyclic peptide Arg 8-vasopressin, which has one disulfide bond, and with bovine insulin, which has three disulfide bonds. A finer isolation was achieved by stored waveform inverse fourier transform (SWIFT), and the selected species were irradiated with electrons for 20 s. The result of the ECD spectrum showed charge reduction to the 3+ and 2+ charge states as well as the loss of a DFC thiyl radical from the 3+ charge state and loss of a DFC molecule from the 2+ charge state.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages719-720
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Cyclotron resonance
Mass spectrometry
Fourier transforms
Gases
Ions
Anti-Bacterial Agents
Disulfides
Peptides
Molecules
Electrons
Pharmaceutical Preparations
Cyclic Peptides
Proteins
Inverse transforms
Vasopressins
Insulin
Plasmas

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Fagerquist, C. K., Hudgins, R. R., Emmett, M., Håkansson, K., & Marshall, A. G. (2002). Selective cleavage of an antibiotic drug molecule from peptides and proteins in the gas phase by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 719-720)

Selective cleavage of an antibiotic drug molecule from peptides and proteins in the gas phase by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry. / Fagerquist, Clifton K.; Hudgins, Robert R.; Emmett, Mark; Håkansson, Kristina; Marshall, Alan G.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 719-720.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fagerquist, CK, Hudgins, RR, Emmett, M, Håkansson, K & Marshall, AG 2002, Selective cleavage of an antibiotic drug molecule from peptides and proteins in the gas phase by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 719-720, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Fagerquist CK, Hudgins RR, Emmett M, Håkansson K, Marshall AG. Selective cleavage of an antibiotic drug molecule from peptides and proteins in the gas phase by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 719-720
Fagerquist, Clifton K. ; Hudgins, Robert R. ; Emmett, Mark ; Håkansson, Kristina ; Marshall, Alan G. / Selective cleavage of an antibiotic drug molecule from peptides and proteins in the gas phase by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 719-720
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