Selective enhancement of the type 1 cytokine response by expression of a canine interleukin (IL)-12 fused heterodimeric DNA

Omar A. Saldarriaga, Luis E. Perez, Bruno L. Travi, Peter C. Melby

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Interleukin-12 (IL-12) is a heterodimeric cytokine that is a principal mediator of the innate immune response and modulator of acquired cell-mediated immunity. Administration of exogenous IL-12 can direct the host adaptive T cell response toward a type 1 phenotype. The co-administration of IL-12 with vaccine antigens has been shown to augment the vaccine-induced TH1 response and protection against intracellular pathogens. We show here that a canine IL-12 DNA, constructed by fusing the p35 and p40 subunit cDNAs with an interspacing linker, generated stable IL-12 transcripts when placed under control of a strong constitutive promoter. The protein expressed from this fused cDNA was fully functional in promoting a type 1 (IFN-γ) and suppressing a type 2 (IL-4) cytokine response following both in vitro transfection of a canine cell line and in vivo delivery to dogs. This DNA construct may be useful as an adjuvant for vaccines that target tumors or intracellular pathogens of the dog.

Original languageEnglish (US)
Pages (from-to)377-388
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume110
Issue number3-4
DOIs
StatePublished - Apr 15 2006

Keywords

  • Canine
  • IFN-γ
  • Interleukin-12
  • Vaccine adjuvant

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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