Selective keratinocyte stimulation is sufficient to evoke nociception in mice

Zixuan Pang, Takashi Sakamoto, Vinod Tiwari, Yu Shin Kim, Fei Yang, Xinzhong Dong, Ali D. Güler, Yun Guan, Michael J. Caterina

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The skin epidermis is densely innervated by peripheral sensory nerve endings. Nociceptive neurons, whose terminals are in close contact with epidermal keratinocytes, can be activated directly by noxious physical and chemical stimuli to trigger pain. However, whether keratinocytes can signal acutely to sensory nerve terminals to initiate pain in vivo remains unclear. Here, using the keratin 5 promoter to selectively express the capsaicin receptor TRPV1 in keratinocytes of TRPV1-knockout mice, we achieved specific stimulation of keratinocytes with capsaicin. Using this approach, we found that keratinocyte stimulation was sufficient to induce strong expression of the neuronal activation marker, c-fos, in laminae I and II of the ipsilateral spinal cord dorsal horn and to evoke acute paw-licking nocifensive behavior and conditioned place aversion. These data provide direct evidence that keratinocyte stimulation is sufficient to evoke acute nociception-related responses.

Original languageEnglish (US)
Pages (from-to)656-665
Number of pages10
JournalPain
Volume156
Issue number4
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

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Nociception
Keratinocytes
Substantia Gelatinosa
Keratin-5
TRPV Cation Channels
Pain
Nociceptors
Capsaicin
Sensory Receptor Cells
Peripheral Nerves
Epidermis
Knockout Mice
Skin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Pang, Z., Sakamoto, T., Tiwari, V., Kim, Y. S., Yang, F., Dong, X., ... Caterina, M. J. (2015). Selective keratinocyte stimulation is sufficient to evoke nociception in mice. Pain, 156(4), 656-665. https://doi.org/10.1097/j.pain.0000000000000092

Selective keratinocyte stimulation is sufficient to evoke nociception in mice. / Pang, Zixuan; Sakamoto, Takashi; Tiwari, Vinod; Kim, Yu Shin; Yang, Fei; Dong, Xinzhong; Güler, Ali D.; Guan, Yun; Caterina, Michael J.

In: Pain, Vol. 156, No. 4, 01.04.2015, p. 656-665.

Research output: Contribution to journalArticle

Pang, Z, Sakamoto, T, Tiwari, V, Kim, YS, Yang, F, Dong, X, Güler, AD, Guan, Y & Caterina, MJ 2015, 'Selective keratinocyte stimulation is sufficient to evoke nociception in mice', Pain, vol. 156, no. 4, pp. 656-665. https://doi.org/10.1097/j.pain.0000000000000092
Pang Z, Sakamoto T, Tiwari V, Kim YS, Yang F, Dong X et al. Selective keratinocyte stimulation is sufficient to evoke nociception in mice. Pain. 2015 Apr 1;156(4):656-665. https://doi.org/10.1097/j.pain.0000000000000092
Pang, Zixuan ; Sakamoto, Takashi ; Tiwari, Vinod ; Kim, Yu Shin ; Yang, Fei ; Dong, Xinzhong ; Güler, Ali D. ; Guan, Yun ; Caterina, Michael J. / Selective keratinocyte stimulation is sufficient to evoke nociception in mice. In: Pain. 2015 ; Vol. 156, No. 4. pp. 656-665.
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