Selective modulation of antioxidant enzyme activities in host tissues during Rickettsia conorii infection

Elena Rydkina, Sanjeev Sahni, Lisa A. Santucci, Loel C. Turpin, Raymond B. Baggs, David J. Silverman

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The involvement of oxidative mechanisms in the pathogenesis of rickettsiosis was investigated using infection of C3H/HeN mice with sub-lethal and lethal infectious doses of Rickettsia conorii, the causative agent of Mediterranean spotted fever. Microscopic examination of tissues at 48 and 96 h post-infection revealed characteristic pathologic features and the presence of rickettsiae in the endothelium of infected tissues. Activities of key antioxidant enzymes, namely glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase, and superoxide dismutase, at these times exhibited a pattern of differential and selective modulation in brain, lungs, and testes of mice infected with viable organisms, whereas heat-inactivated or sonically disrupted rickettsiae had no effect. Of these, most significant changes were evident in the lungs of infected animals. Adaptive alterations in oxidant-scavenging enzymes occurred in apparent correlation with the dose and duration of infection. Treatment with an antioxidant, α-lipoic acid, protected against infection-induced oxidative injury via regulation of antioxidant enzyme activities and maintenance of reduced glutathione levels. These results suggest the involvement of regulatory enzymes of glutathione redox and superoxide scavenging systems in the antioxidant response during in vivo infection, the extent of which varies with the titer of viable rickettsiae in different organs of the host.

Original languageEnglish (US)
Pages (from-to)293-301
Number of pages9
JournalMicrobial Pathogenesis
Volume36
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Rickettsia Infections
Rickettsia conorii
Rickettsia
Antioxidants
Enzymes
Infection
Glutathione
Boutonneuse Fever
Thioctic Acid
Lung
Glutathione Reductase
Inbred C3H Mouse
Glucosephosphate Dehydrogenase
Glutathione Peroxidase
Oxidants
Superoxides
Superoxide Dismutase
Oxidation-Reduction
Endothelium
Testis

Keywords

  • Antioxidant
  • Endothelium
  • Pathogenesis
  • Rickettsia conorii
  • Rickettsiosis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Selective modulation of antioxidant enzyme activities in host tissues during Rickettsia conorii infection. / Rydkina, Elena; Sahni, Sanjeev; Santucci, Lisa A.; Turpin, Loel C.; Baggs, Raymond B.; Silverman, David J.

In: Microbial Pathogenesis, Vol. 36, No. 6, 06.2004, p. 293-301.

Research output: Contribution to journalArticle

Rydkina, Elena ; Sahni, Sanjeev ; Santucci, Lisa A. ; Turpin, Loel C. ; Baggs, Raymond B. ; Silverman, David J. / Selective modulation of antioxidant enzyme activities in host tissues during Rickettsia conorii infection. In: Microbial Pathogenesis. 2004 ; Vol. 36, No. 6. pp. 293-301.
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