Selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40

Mariano Garcia-Blanco, P. K. Ghosh, B. M. Jayaram, S. Ivory, P. Lebowitz, P. Lengyel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Treatment of African green monkey kidney CV-1 cells with human alpha interferons before infection with simian virus 40 (SV40) inhibited the accumulation of SV40 mRNAs and SV40 T-antigen (Tag). This inhibition persisted as long as the interferons were present in the medium. SV40-transformed human SV80 cells and mouse SV3T3-38 cells express Tag, and interferon treatment of these cells did not affect this expression. SV80 and SV3T3-38 cells which had been exposed to interferons were infected with a viable SV40 deletion mutant (SV40 dl1263) that codes for a truncated Tag. Exposure to interferons inhibited the accumulation of the truncated Tag (specified by the infecting virus) but had no significant effect on the accumulation of the endogenous Tag (specified by the SV40 DNA integrated into the cellular genome). The level of Tag in SV40-transformed mouse SV101 cells was not significantly decreased by interferon treatment. SV40 was rescued from SV101 cells and used to infect interferon-treated and control African green monkey kidney Vero cells. Tag accumulation was inhibited in the cells which had been treated with interferons before infection. Our data demonstrate that even within the same cell the interferon system can discriminate between expression of a gene in the SV40 viral genome and expression of the same gene integrated into a host chromosome.

Original languageEnglish (US)
Pages (from-to)893-898
Number of pages6
JournalJournal of Virology
Volume53
Issue number3
StatePublished - 1985
Externally publishedYes

Fingerprint

Simian virus 40
interferons
Interferons
Viral Tumor Antigens
antigens
cells
Cercopithecus aethiops
kidneys
Kidney
Gene Expression
gene expression
interferon-alpha
Vero Cells
genome
Viral Genome
mice
Infection
Interferon-alpha
infection
T-lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Garcia-Blanco, M., Ghosh, P. K., Jayaram, B. M., Ivory, S., Lebowitz, P., & Lengyel, P. (1985). Selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40. Journal of Virology, 53(3), 893-898.

Selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40. / Garcia-Blanco, Mariano; Ghosh, P. K.; Jayaram, B. M.; Ivory, S.; Lebowitz, P.; Lengyel, P.

In: Journal of Virology, Vol. 53, No. 3, 1985, p. 893-898.

Research output: Contribution to journalArticle

Garcia-Blanco, M, Ghosh, PK, Jayaram, BM, Ivory, S, Lebowitz, P & Lengyel, P 1985, 'Selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40', Journal of Virology, vol. 53, no. 3, pp. 893-898.
Garcia-Blanco, Mariano ; Ghosh, P. K. ; Jayaram, B. M. ; Ivory, S. ; Lebowitz, P. ; Lengyel, P. / Selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40. In: Journal of Virology. 1985 ; Vol. 53, No. 3. pp. 893-898.
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