Self-assembly of nucleocapsid-like particles from recombinant hepatitis C virus core protein

M. Kunkel, M. Lorinczi, R. Rijnbrand, S. M. Lemon, Stanley Watowich

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Little is known about the assembly pathway and structure of hepatitis C virus (HCV) since insufficient quantities of purified virus are available for detailed biophysical and structural studies. Here, we show that bacterially expressed HCV core proteins can efficiently self-assemble in vitro into nucleocapsid-like particles. These particles have a regular, spherical morphology with a modal distribution of diameters of approximately 60 nm. Self-assembly of nucleocapsid-like particles requires structured RNA molecules. The 124 N-terminal residues of the core protein are sufficient for self-assembly into nucleocapsid-like particles. Inclusion of the carboxy-terminal domain of the core protein modifies the core assembly pathway such that the resultant particles have an irregular outline. However, these particles are similar in size and shape to those assembled from the 124 N-terminal residues of the core protein. These results provide novel opportunities to delineate protein-protein and protein-RNA interactions critical for HCV assembly, to study the molecular details of HCV assembly, and for performing high-throughput screening of assembly inhibitors.

Original languageEnglish (US)
Pages (from-to)2119-2129
Number of pages11
JournalJournal of Virology
Volume75
Issue number5
DOIs
StatePublished - 2001

Fingerprint

Nucleocapsid
nucleocapsid
Hepatitis C virus
Hepacivirus
Virus Assembly
Proteins
proteins
RNA
Hepatitis C virus nucleocapsid protein
Viruses
screening
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Self-assembly of nucleocapsid-like particles from recombinant hepatitis C virus core protein. / Kunkel, M.; Lorinczi, M.; Rijnbrand, R.; Lemon, S. M.; Watowich, Stanley.

In: Journal of Virology, Vol. 75, No. 5, 2001, p. 2119-2129.

Research output: Contribution to journalArticle

Kunkel, M. ; Lorinczi, M. ; Rijnbrand, R. ; Lemon, S. M. ; Watowich, Stanley. / Self-assembly of nucleocapsid-like particles from recombinant hepatitis C virus core protein. In: Journal of Virology. 2001 ; Vol. 75, No. 5. pp. 2119-2129.
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