Self-oligomerization regulates stability of survival motor neuron protein isoforms by sequestering an SCFSlmb degron

Kelsey M. Gray, Kevin A. Kaifer, David Baillat, Ying Wen, Thomas R. Bonacci, Allison D. Ebert, Amanda C. Raimer, Ashlyn M. Spring, Sara ten Have, Jacqueline J. Glascock, Kushol Gupta, Gregory D. Van Duyne, Michael J. Emanuele, Angus I. Lamond, Eric Wagner, Christian L. Lorson, A. Gregory Matera

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1. Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMN?7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of survival motor neuron (SMN) isoforms are poorly understood. We used a Drosophila model system and label-free proteomics to identify the SCFSlmb ubiquitin E3 ligase complex as a novel SMN binding partner. SCFSlmb interacts with a phosphor degron embedded within the human and fruitfly SMN YG-box oligomerization domains. Substitution of a conserved serine (S270A) interferes with SCFSlmb binding and stabilizes SMN?7. SMA-causing missense mutations that block multimerization of full-length SMN are also stabilized in the degron mutant background. Overexpression of SMN?7S270A, but not wild-type (WT) SMN?7, provides a protective effect in SMA model mice and human motor neuron cell culture systems. Our findings support a model wherein the degron is exposed when SMN is monomeric and sequestered when SMN forms higher-order multimers.

Original languageEnglish (US)
Pages (from-to)96-110
Number of pages15
JournalMolecular Biology of the Cell
Volume29
Issue number2
DOIs
StatePublished - Jan 15 2018

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Motor Neurons
Protein Isoforms
Spinal Muscular Atrophy
Exons
Duplicate Genes
Ubiquitin-Protein Ligases
Missense Mutation
Proteomics
Serine
Drosophila
Cell Culture Techniques
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Gray, K. M., Kaifer, K. A., Baillat, D., Wen, Y., Bonacci, T. R., Ebert, A. D., ... Gregory Matera, A. (2018). Self-oligomerization regulates stability of survival motor neuron protein isoforms by sequestering an SCFSlmb degron. Molecular Biology of the Cell, 29(2), 96-110. https://doi.org/10.1091/mbc.E17-11-0627)

Self-oligomerization regulates stability of survival motor neuron protein isoforms by sequestering an SCFSlmb degron. / Gray, Kelsey M.; Kaifer, Kevin A.; Baillat, David; Wen, Ying; Bonacci, Thomas R.; Ebert, Allison D.; Raimer, Amanda C.; Spring, Ashlyn M.; Have, Sara ten; Glascock, Jacqueline J.; Gupta, Kushol; Van Duyne, Gregory D.; Emanuele, Michael J.; Lamond, Angus I.; Wagner, Eric; Lorson, Christian L.; Gregory Matera, A.

In: Molecular Biology of the Cell, Vol. 29, No. 2, 15.01.2018, p. 96-110.

Research output: Contribution to journalArticle

Gray, KM, Kaifer, KA, Baillat, D, Wen, Y, Bonacci, TR, Ebert, AD, Raimer, AC, Spring, AM, Have, ST, Glascock, JJ, Gupta, K, Van Duyne, GD, Emanuele, MJ, Lamond, AI, Wagner, E, Lorson, CL & Gregory Matera, A 2018, 'Self-oligomerization regulates stability of survival motor neuron protein isoforms by sequestering an SCFSlmb degron', Molecular Biology of the Cell, vol. 29, no. 2, pp. 96-110. https://doi.org/10.1091/mbc.E17-11-0627)
Gray, Kelsey M. ; Kaifer, Kevin A. ; Baillat, David ; Wen, Ying ; Bonacci, Thomas R. ; Ebert, Allison D. ; Raimer, Amanda C. ; Spring, Ashlyn M. ; Have, Sara ten ; Glascock, Jacqueline J. ; Gupta, Kushol ; Van Duyne, Gregory D. ; Emanuele, Michael J. ; Lamond, Angus I. ; Wagner, Eric ; Lorson, Christian L. ; Gregory Matera, A. / Self-oligomerization regulates stability of survival motor neuron protein isoforms by sequestering an SCFSlmb degron. In: Molecular Biology of the Cell. 2018 ; Vol. 29, No. 2. pp. 96-110.
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abstract = "Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1. Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMN?7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of survival motor neuron (SMN) isoforms are poorly understood. We used a Drosophila model system and label-free proteomics to identify the SCFSlmb ubiquitin E3 ligase complex as a novel SMN binding partner. SCFSlmb interacts with a phosphor degron embedded within the human and fruitfly SMN YG-box oligomerization domains. Substitution of a conserved serine (S270A) interferes with SCFSlmb binding and stabilizes SMN?7. SMA-causing missense mutations that block multimerization of full-length SMN are also stabilized in the degron mutant background. Overexpression of SMN?7S270A, but not wild-type (WT) SMN?7, provides a protective effect in SMA model mice and human motor neuron cell culture systems. Our findings support a model wherein the degron is exposed when SMN is monomeric and sequestered when SMN forms higher-order multimers.",
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